Study on relationship of target organ injury of mechanism and

Sha-sha Zhao; Hai HE; Zi-yang Wang; Yao-ying Xing; Yuan Ren; Jing Shao; Sha-sha Zhao; Hai HE; Zi-yang Wang; Yao-ying Xing; Jing Shao; Yuan Ren; Jing Shao; Jing Shao

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Study on relationship of target organ injury of mechanism and "structure-effect-dose" of Hedysari Radix during radiotherapy-chemotherapy induced

Author: Sha-Sha ZHAO ¹ ; Hai HE ¹ ; Zi-Yang WANG ¹ ; Yao-Ying XING ¹ ; Yuan REN ¹ ; Jing SHAO ¹ ; Sha-Sha ZHAO ² ; Hai HE ² ; Zi-Yang WANG ² ; Yao-Ying XING ² ; Jing SHAO ² ; Yuan REN ³ ; Jing SHAO ³ ; Jing SHAO ⁴
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1. Gansu University of Traditional Chinese Medicine
2. The Co-construction and Collaborative Innovation Center of Northwest Chinese and Tibetan Medicine Ministry
3. Key Laboratory of Pharmacology and Toxicology of Traditional Chinese Medicine of Gansu Province
4. Provincial Key Laboratory of Chinese (Tibet) Medicine Chemistry and Quality Research of Higher Education Institutions in Gansu Province
Publication Type:Journal Article
Keywords: HPLC; molecular docking; network pharmacology; radiotherapy and chemotherapy; Radix Hedysari; target organ damage
From: Chinese Pharmacological Bulletin 2024;40(2):371-380
CountryChina
Language:Chinese
Abstract: Aim To explore the possible mechanism of "component-target-pathway" of Radix Hedysari against target organ damage caused by radiotherapy and chemotherapy, and to verify the " dose-effect" relationship of the main active components. Methods TCMSP, Uniprot, Swiss Target Prediction, GeneCards, Cytoscape, Omicshare and other platforms were used for network pharmacology analysis. Autodock, Pymol and Ligplot were used for molecular docking. The water extract of Radix Hedysari was used for animal experiment verification. The contents of eight main components were determined by HPLC. Results Four active components, eight key targets and four key pathways of Radix Hedysari were identified to resist the damage of target organs caused by radiotherapy and chemotherapy. Molecular docking showed that formononetin and quercetin had good binding activity with HSP90AA1, naringenin and MAPK3, and ursolic acid and TP53. Animal experiments showed that gastrointestinal factors MTL and VIP increased significantly, liver and kidney factors Cr, BUN, AST and ALT decreased significantly, inflammatory factor IL-10 increased significantly and TNF-a decreased significantly. The content of ononm was the highest (2 . 884 8 µg • g "