Influence of quercetin on aging of bone marrow mesenchymal stem cells induced by microgravity
- Author:
Yu-Tian YANG
1
;
Ying-Ying XUAN
1
;
Yu-Tian YANG
2
;
Ying-Ying XUAN
2
;
Yu-Hai GAO
2
;
Long-Fei WANG
2
;
Han-Qin TANG
2
;
Zhi-Hui MA
2
;
Liang LI
2
;
Yi WU
2
;
Ke-Ming CHEN
2
;
Yu-Tian YANG
3
;
Ying-Ying XUAN
3
;
Yu-Hai GAO
3
;
Long-Fei WANG
3
;
Han-Qin TANG
3
;
Zhi-Hui MA
3
;
Liang LI
3
;
Yi WU
3
;
Ke-Ming CHEN
3
Author Information
- Publication Type:Journal Article
- Keywords: aging; bone marrow stromal cells; lamin B1; microgravity; mito-chondrion; oxidative stress; quercetin
- From: Chinese Pharmacological Bulletin 2024;40(1):38-45
- CountryChina
- Language:Chinese
- Abstract: Aim To investigate the effect of quercetin on the aging model of bone marrow mesenchymal stem cells established under microgravity. Methods Using 3D gyroscope, a aging model of bone marrow mesenchymal stem cells was constructed, and after receiving quercetin and microgravity treatment, the anti-aging effect of the quercetin was evaluated by detecting related proteins and oxidation indexes. Results Compared to the control group, the expressions of age-related proteins p21, pi6, p53 and RB in the microgravity group significantly increased, while the expressions of cyclin D1 and lamin B1 significantly decreased, with statistical significance (P<0.05). In the microgravity group, mitochondrial membrane potential significantly decreased (P<0.05), ROS accumulation significantly increased (P <0.05), SOD content significantly decreased and MDA content significantly increased (P<0.05). Compared to the microgravity group, the expressions of age-related proteins p21, pi6, p53 and RB in the quercetin group significantly decreased, while the expressions of cyclin D1 and lamin B1 significantly increased, with statistical significance (P<0.05). In the quercetin group, mitochondrial membrane potential significantly increased (P<0.05), ROS accumulation significantly decreased (P<0.05), SOD content significantly increased and MDA content significantly decreased (P<0.05). Conclusions Quercetin can resist oxidation, protect mitochondrial function and normal cell cycle, thus delaying the aging of bone marrow mesenchymal stem cells induced by microgravity.
