Resistance to deltamethrin and its association with mutation sites in the sodium iron channel domain III gene in Rhipicephalus microplus in Huaihua City of Hunan Province
10.16250/j.32.1374.2023113
- VernacularTitle:湖南省怀化地区微小扇头蜱溴氰菊酯抗药性及与钠 离子通道结构域Ⅲ基因突变位点的关联研究
- Author:
Zhongbo LI
1
,
2
,
3
;
Tian YANG
1
;
Ming SHU
1
;
Hui HU
1
;
Cuiqin HUANG
3
,
4
Author Information
1. Huaihua Vocational and Technical College, Huaihua, Hunan 418000, China
2. College of Life Science, Longyan University, Longyan, Fujian 364012, China
3. Engineering Research Center for Prevention and Control of Animal Original Zoonosis, Fujian Province University, Longyan, Fujian 364012, China
4. College of Life Science, Longyan University, Longyan, Fujian 364012, China
- Publication Type:Journal Article
- Keywords:
Rhipicephalus microplus;
Deltamethrin;
Sodium ion channel domain III gene;
Adult immersion test;
Mutation site;
Drug resistance
- From:
Chinese Journal of Schistosomiasis Control
2024;36(1):17-24
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the level of deltamethrin resistance and mutation sites in the sodium iron channel gene in Rhipicephalus microplus in Huaihua City, Hunan Province, and to examine the correlation between deltamethrin resistance and mutation sites in the sodium iron channel gene in Rh. microplus. Methods Rh. microplus was sampled from multiple yellow cattle farms in Huaihua City, Hunan Province from June to September 2022, and the level of resistance to deltamethrin was determined in ticks using the adult immersion test. The sodium iron channel domain III gene was amplified in deltamethrin-resistant and wild-type Rh. microplus using PCR assay. Following sequencing and sequence alignment, mutation sites were detected in bases. The sodium iron channel domain III gene in Rh. microplus was translated, and the signal peptide, transmembrane domain, and phosphorylation and glycosylation sites were detected in amino acid sequences. The tertiary structures of the sodium iron channel domain III protein of deltamethrin-resistant and wild-type Rh. microplus were deduced and compared, and the association be tween mutation sites in bases and resistance to deltamethrin was examined in Rh. microplus according the level of deltamethrin resistance, sequence alignment and protein tertiary structure. Results The median (LC50) and 95% lethal concentrations (LC95) of deltamethrin were 121.39 mg/L and 952.61 mg/L against Rh. microplus, with a resistance factor of 9.24 and level II resistance. The sequence of the sodium ion channel domain III gene was 1 010 bp in size, and mutation sites were detected in two neighboring bases in the sequence of the sodium ion channel domain III gene in deltamethrin-resistant Rh. microplus. Although no signal peptides were found in the sodium iron channel domain III protein of deltamethrin-resistant or wild-type Rh. microplus, 6 trans-membrane domains, 42 phosphorylation sites and 8 glycosylation sites were identified, with a significant difference in the tertiary structure of the sodium iron channel domain III protein between deltamethrin-resistant and wild-type Rh. microplus. Conclusions Level II resistance to deltamethrin is detected in Rh. microplus in Huaihua City, Hunan Province, and two mutation sites that correlate with the emergence of deltamethrin resistance are identified in the sequence of the sodium iron channel domain III gene in deltamethrin-resistant Rh. microplus.
