Establishment of HPLC fingerprint and content determination of multiple index components in Xiao’er resuqing oral liquid
- VernacularTitle:小儿热速清口服液HPLC指纹图谱的建立及多指标成分的含量测定
- Author:
Junfeng CUI
1
,
2
;
Shuai CUI
1
,
2
;
Xiukun LIANG
1
,
2
;
Dongxu LI
1
,
2
;
Xinrui WANG
1
,
2
;
Peng GAO
3
Author Information
1. Binzhou Testing Center，Shandong Binzhou 256600，China
2. Binzhou Key Laboratory of Chemical Drug Research and Quality Control，Shandong Binzhou 256600，China
3. College of Pharmacy，Shandong University of Traditional Chinese Medicine，Jinan 250355，China
- Publication Type:Journal Article
- Keywords:
Xiao’er resuqing oral liquid;
HPLC;
fingerprint;
content determination
- From:
China Pharmacy
2024;35(7):801-806
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To establish an HPLC fingerprint of Xiao’er resuqing oral liquid， and to determine the contents of twelve index components. METHODS HPLC method was adopted. The determination was performed on Venusil MP C18 column with mobile phase consisting of acetonitrile-0.1% phosphate aqueous solution （gradient elution） at a flow rate of 1.0 mL/min. The detection wavelength was set at 210 nm， the column temperature was 30 ℃， the injection volume was 10 μL. HPLC fingerprint of Xiao’er resuqing oral liquid was established by using the Similarity Evaluation System of Chromatographic Fingerprint of TCM （2012 edition） to evaluate the similarity. The contents of 12 components were determined， including （R， S）-goitrin， 3，5-O-dicaffeoyl quinic acid， puerarin， forsythin， forsythoside A， chlorogenic acid， baicalin， saikosaponins d， wogonoside， baicalein， emodin and chrysophanol. RESULTS The similarity of HPLC fingerprints of 13 batches of Xiao’er resuqing oral liquid was greater than 0.97， and 14 common peaks were confirmed. The contents of the above 12 index components in 13 batches of Xiao’er resuqing oral liquid were as follows： 0.078-0.172， 1.564-2.736， 1.338-2.578， 0.426-0.872， 1.477-2.628， 1.396-2.447， 4.052-9.146， 0.367- 0.692， 1.974-4.674， 1.274-2.969， 0.085-0.167 and 0.155-0.307 mg/mL. CONCLUSIONS The established HPLC fingerprint and content determination methods have high accuracy and high specificity， which can be used for the quality evaluation of Xiao’er resuqing oral liquid.
