Effect of Garcinia binucao crude leaf extract supplementation on lifespan of Drosophila melanogaster chronically exposed to alcohol
https://doi.org/10.47895/amp.vi0.5911
- Author:
Joanne Jennifer E. Tan
1
;
Ourlad Alzeus G. Tantengco
1
;
Nicholas Robert C. Tan
1
;
Clyde E. Silverio
1
;
Ana Denise V. Sison
1
;
Joseph P. Sta. Maria Jr.
1
;
Karol Ina G. Tablante
1
;
Joyce Gillian A. Tiam-Lee
1
;
Maria Concepcion C. Sison
2
;
Paul Mark B. Medina
3
Author Information
1. College of Medicine, University of the Philippines Manila
2. Department of Pharmacology and Toxicology, College of Medicine, University of the Philippines Manila
3. Department of Biochemistry and Molecular Biology, College of Medicine, University of the Philippines Manila
- Publication Type:Journal Article
- Keywords:
Garcinia binucao;
Chronic alcohol toxicity
- MeSH:
Drosophila melanogaster;
Longevity
- From:
Acta Medica Philippina
2024;58(5):52-56
- CountryPhilippines
- Language:English
-
Abstract:
Background:Consumption and abuse of alcohol remains a significant cause of concern worldwide. Furthermore, there is evidence of the association between chronic alcohol use and reduced life expectancy.
Objectives:To study the effects of Garcinia binucao extract (GBE) supplementation on lifespan of Drosophila
melanogaster, in the presence or absence of chronic alcohol exposure.
Methods:D. melanogaster was mass cultured and given GBE supplementation in high (1 mg/mL) and low (200 µg/mL) sublethal doses. D. melanogaster flies were divided into groups - with and without chronic alcohol exposure, and their respective lifespans were monitored.
Results:In D. melanogaster without alcohol exposure, mean lifespan was highest in the control flies (38.15 days), followed by high-dose GBE (34.42 days), low-dose GBE (33.24 days), and DMSO (22.29 days). In D. melanogaster chronically exposed to alcohol, the longest mean lifespan was observed in flies treated with high-dose GBE (33.80 days), followed by low-dose GBE (33.63 days), the DMSO group (30.30), and the control group (29.65 days), but the differences were not statistically significant. Comparing groups with and without chronic alcohol exposure, the mean lifespan of the control group chronically exposed to alcohol significantly decreased by 9.51 days (p < 0.05). In GBE treatment groups, mean lifespan significantly decreased by 0.82 days in high-dose set-up (p < 0.05), and significantly increased by 0.39 days in the low-dose set-up (p < 0.05) upon chronic alcohol exposure.
Conclusion:Garcinia binucao extract supplementation ameliorated the observed reduction in lifespan of Drosophila melanogaster chronically exposed to alcohol.
- Full text:5911-Article Text-106429-2-10-20240321 (1).pdf
