Effect of enriched environment combined with acupuncture at head points on behavior in rats with autism spectrum disorder
10.3969/j.issn.1006-9771.2024.02.006
- VernacularTitle:丰富环境训练结合头穴丛刺对孤独症谱系障碍模型大鼠行为的影响
- Author:
Zichen MU
1
;
Qiang TANG
2
;
Yunqiu SHI
1
;
Yan WANG
2
;
Shuwei ZHU
2
;
Ya'nan ZHUANG
2
;
Danshuang XU
2
;
Hongyu LI
2
;
Baolong LI
1
;
Chunyan ZHANG
1
;
Mengke YUAN
1
Author Information
1. Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang 150040, China
2. the Second Affiliated Hospital of Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang 150001, China
- Publication Type:Journal Article
- Keywords:
autism spectrum disorder;
acupuncture at head point;
enriched environment;
behavior;
rats
- From:
Chinese Journal of Rehabilitation Theory and Practice
2024;30(2):176-182
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo observe the effect of enriched environment (EE) combined with acupuncture at head point (HA) on behavior in rats with autism spectrum disorder. MethodsHealthy female Wistar rats were given peritoneal injection of sodium valproate at 12.5 days of gestation. Twenty-four male offspring rats were randomly selected and then randomly divided into model group (n = 6), EE group (n = 6), HA group (n = 6) and EE combined with HA group (the combined group, n = 6). Six male offspring rats born from female mice injected with the same amount of saline intraperitoneally were as control group. After four weeks of treatment, all the five groups were tested with three-chamber test and marble burying test, and the sociability index, the social novelty index and the number of buried marbles were recorded. The levels of interleukin (IL)-1β and IL-6 in peripheral blood were determined by enzyme-linked immunosorbent assay (ELISA). ResultsAfter treatment, compared with the model group, the sociability index and the social novelty index improved (P < 0.05), the number of buried marbles reduced (P < 0.05), and the levels of IL-6 and IL-1β in peripheral blood decreased in EE group, HA group and the combined group (P < 0.05); while the combined group was the best (P < 0.01). ConclusionBoth EE or acupuncture at HA could improve behavioral symptoms, and reduce the expression of inflammatory factors in rats with autism spectrum disorder. The combination of the two methods showed the best result.
