Determination of four organotin compounds in human whole blood by high performance liquid chromatography-inductively coupled plasma-mass spectrometry
10.20001/j.issn.2095-2619.20231219
- VernacularTitle:高效液相色谱-电感耦合等离子体-质谱法测定人全血中4种有机锡化合物
- Author:
Anping MA
1
;
Banghua WU
;
Ming DONG
;
Xiaolin RUAN
;
Jingjing QIU
;
Weifeng RONG
;
Jiaheng HE
Author Information
1. Guangdong Province Hospital for Occupational Disease Prevention and Treatment, Guangzhou, Guangdong 510300, China
- Publication Type:Journal Article
- Keywords:
High performance liquid chromatography;
Inductively coupled plasma-mass spectrometry;
Organotin compounds;
Whole Blood
- From:
China Occupational Medicine
2023;50(6):712-716
- CountryChina
- Language:Chinese
-
Abstract:
{L-End}Objective To establish a method for the simultaneous determination of dimethyltin (DMT), trimethyltin (TMT), diethyltin (DET), and triethyltin (TET) in human whole blood using high performance liquid chromatography-inductively coupled plasma-mass spectrometry (ICP-MS). {L-End}Methods The 1.0 mL of blood was added with 4.0 mL 65% aqueous solution (containing 6% acetic acid), extracted and separated by C4 column (150 mm×3 mm×3 μm) using a mobile phase of methanol and 4% acetic acid aqueous solution (containing 0.25 mmol/L tropolone) at a volume ratio of 35∶65, and detected by ICP-MS. {L-End}Results The linear range of DMT, TMT, DET, and TET was 30.60-550.80, 29.00-522.00, 46.10-829.80, and 34.05-612.90 μg/L, respectively. All correlation coefficients were 0.999. The detection limit of DMT, TMT, DET and TET was 21.40, 20.30, 32.27 and 23.80 μg/L, respectively. The recovery rate was 81.9%-104.9%. The within-run and between-run relative standard deviation was 1.6%-6.9% and 0.1%-10.0%, respectively. The samples can be stored at -20 ℃ and 4 ℃ for at least three days. {L-End}Conclusion This method can be used for trace analysis of DMT, TMT, DET, and TET in whole blood.
