Content measurement of doxorubicin hydrochloride and lonidamine by HPLC
10.12206/j.issn.2097-2024.202306043
- VernacularTitle:HPLC法同时测定盐酸阿霉素与氯尼达明的含量
- Author:
Yuhan SUN
1
;
Ziyi XU
1
;
Jun LIAO
2
;
He ZHANG
1
;
Li FAN
1
;
Ying LU
1
Author Information
1. Department of Pharmaceutical, School of Pharmacy, Naval Medical University, Shanghai 200433, China.
2. School of Medicine, Shanghai University, Shanghai 200444, China.
- Keywords:
lonidamine;
doxorubicin hydrochloride;
HPLC;
drug combination;
gradient elution
- From:
Journal of Pharmaceutical Practice and Service
2024;42(3):127-130
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for the simultaneous determination of DOX·HCl and LND. Methods HPLC was performed on Agilent 5 HC-C18（2） （4.6 mm × 250 mm, 5 µm） column. The mobile phase was methanol-0.1% TFA aqueous solution, and the gradient elution procedure were: 0 to 3 min, 65% methanol; 3 to 7 min, 65%→90% methanol; 7 to 13 min, 90% methanol; 13 to 15 min, 90%→65% methanol; 15 to 20 min, 65% methanol. The collection time was 20 min, the balance time was 3 min, the UV detection wavelengths were 205 nm and 253 nm. The flow rate was 1.0 ml/min and the column temperature was 35℃. The amount of inlet was 10 µl. Results The method was highly specific, and both DOX·HCl and LND exhibited good linearity in the concentration range of 1-40 µg/ml and 6-240 µg/ml, respectively. The two compounds’ precision, stability, and recovery satisfied the requirements of the method. Conclusion This study established a HPLC method that was suitable for the simultaneous detection of DOX·HCl and LND. This method’s high level of specificity, accuracy, and reliability .
