Establishment of a high-throughput screening platform based on drug repurposing targeting alpha-1-acid glycoprotein and discovery of potential weight loss drugs
10.12206/j.issn.2097-2024.202309057
- VernacularTitle:基于药物重定位建立以α1酸性糖蛋白为靶点的高通量筛选平台及潜在减肥药物的发现
- Author:
Feng CHEN
1
;
Cirong YANG
2
;
Zhen ZHANG
1
;
Fei CHEN
1
;
Xia LIU
1
Author Information
1. School of Pharmacy, Naval Medical University, Shanghai 200433, China.
2. Unit 92, 92730 Force, Sanya 572000, China.
- Keywords:
drug repurposing;
ORM;
high-throughput screening;
obesity;
weight loss drugs
- From:
Journal of Pharmaceutical Practice and Service
2024;42(3):114-120
- CountryChina
- Language:Chinese
-
Abstract:
Objective Alpha-1-acid glycoprotein （ORM） was a new target for the development of weight loss drugs. To search for potential weight loss drugs that could target ORM from the compound library of already marketed drugs based on drug repurposing. Methods The pGL4.20-ORM1 promoter recombinant plasmid was contructed and validated, and then a lentiviral vector was utilized to establish stable AML12 cell lines expressing ORM1 promoter-LUC-PURO. This cell line was employed for high-throughput screening of compounds from the marketed drug library, and the luminescence value of the cells was characterized by enzyme marker. Results Primary screening and secondary screening of 1 470 compounds identified 42 compounds that increased ORM1 promoter expression and could be used for further weight loss effect assessment. Conclusion This study successfully constructed LV-AML12-ORM1 promoter-LUC-PURO stable expression cell lines using lentiviral vectors, laying a foundation for efficient and stable screening of weight loss drugs targeting ORM.
