Effects of fractionated low-dose ionizing radiation in the induction of EA.hy926 cell senescence
10.13491/j.issn.1004-714X.2024.01.003
- VernacularTitle:分次低剂量电离辐射在诱导EA.hy926细胞衰老中的作用
- Author:
Yashi CAI
1
,
2
;
Weixu HUANG
2
;
Lingyu ZHANG
3
,
4
;
Min ZHANG
2
;
Huixian LI
2
;
Changyong WEN
2
;
Zhini HE
5
;
Jianming ZOU
2
;
Huifeng CHEN
1
,
2
Author Information
1. School of Public Health, Southern Medical University, Guangzhou 510515 China
2. Guangdong Province Hospital for Occupational Disease Pevention and Treatment, Guangzhou 510300 China.
3. Guangdong Province Hospital for Occupational Disease Pevention and Treatment, Guangzhou 510300 China
4. School of Public Health, Guangzhou Medical University, Guangzhou 511436 China.
5. School of Public Health, Southern Medical University, Guangzhou 510515 China.
- Publication Type:OriginalArticles
- Keywords:
Low-dose ionizing radiation;
Oxidative stress;
DNA damage;
Cellular senescence
- From:
Chinese Journal of Radiological Health
2024;33(1):13-20
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanism of fractionated low-dose ionizing radiation (LDIR) in the induction of EA.hy926 cell senescence. Methods EA.hy926 cells were irradiated with X-ray at 0, 50, 100, and 200 mGy × 4, respectively, and cultured for 24, 48, and 72 h. Several indicators were measured, including the levels of cellular senescence-associated β-galactosidase (SA-β-gal) staining, mRNA levels of senescence-associated cell cycle protein-dependent kinase inhibitor genes CDKN1A and CDKN2A, reactive oxygen species (ROS), total antioxidant capacity (T-AOC), and phosphorylated H2A histone family member X (γ-H2AX). Results After 4 fractionated LDIR, compared with the control group, the treatment groups showed increased nucleus area, blurred cell edge, and increased SA-β-gal positive area (P < 0.05) at 24, 48 and 72 h. After 4 fractionated LDIR, the mRNA level of CDKN1A increased in the 100 and 200 mGy × 4 groups at 24 and 48 h (P < 0.05), and CDKN2A mRNA level increased in the 100 and 200 mGy × 4 groups at 48 and 72 h (P < 0.05). The fluorescence intensity of ROS increased in treatment groups at 24, 48, and 72 h after 4 fractionated LDIR (P < 0.05). After 4 fractionated LDIR, the T-AOC level increased in the 100 and 200 mGy × 4 groups at 24 h (P < 0.05), and T-AOC level increased in all treatment groups at 48 and 72 h (P < 0.05). After 4 fractionated LDIR, γ-H2AX fluorescence intensity increased in all treatment groups at 24 h (P < 0.05), and the fluorescence intensity increased in the 100 and 200 mGy × 4 groups at 48 and 72 h (P < 0.05). Conclusion Fractionated LDIR can induce cellular senescence in EA.hy926 cells by impacting the cellular oxidation-antioxidation and oxidative damage levels, and the effects were relatively evident at 100 and 200 mGy.
