Establishment of specific chromatogram，chemical pattern recognition analysis and identification with different origins and counterfeit products of Uncariae Ramulus Cum Uncis
- VernacularTitle:钩藤药材的特征图谱建立、化学模式识别分析及其与不同基原、混伪品的鉴别
- Author:
Yuqing HE
1
,
2
,
3
,
4
;
Shengjun CHEN
2
,
3
,
4
;
Haiqin ZHOU
2
,
3
,
4
;
Run QIAN
2
,
3
,
4
;
Chao GU
2
,
3
,
4
;
Simei XIE
1
,
2
,
3
,
4
;
Hongmei WEN
1
Author Information
1. School of Pharmacy，Nanjing University of Chinese Medicine，Nanjing 210023，China
2. Jiangyin Tianjiang Pharmaceutical Co.，Ltd. Technical Center，Jiangsu Jiangyin 214434，China
3. Key Laboratory of Key Technologies for Preparation and Quality Control of Traditional Chinese Medicine Formula Granules in Jiangsu Province，Jiangsu Jiangyin 214434，China
4. Jiangsu Provincial Traditional Chinese Medicine Formula Granule Engineering Technology Center，Jiangsu Jiangyin 214434，China
- Publication Type:Journal Article
- Keywords:
Uncariae Ramulus Cum Uncis;
ultra-high
- From:
China Pharmacy
2024;35(5):566-571
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To establish the ultra-high liquid chromatography （UPLC） characteristic spectrum of Uncariae Ramulus Cum Uncis from different producing areas， to conduct chemical pattern recognition analysis， and to identify the medicinal materials of their different origins and counterfeit products. METHODS UPLC method was adopted to establish the characteristic spectra of 43 batches of Uncariae Ramulus Cum Uncis from different origins； cluster analysis combined with principal component analysis were used to analyze their quality； Uncariae Ramulus Cum Uncis from different origins and counterfeit products were identified. RESULTS UPLC specific spectrum of Uncariae Ramulus Cum Uncis was established， and 13 common peaks were calibrated； peak 2 was identified as catechin， peak 3 as chlorogenic acid， peak 4 as cryptochlorogenic acid， peak 7 as isochlorogenic acid B， peak 8 as isodehydroguotenine， peak 9 as isooguotenine， peak 10 as dehydroguotenine， peak 11 as isochlorogenic acid C， peak 12 as goutenine， and peak 13 as camptothecin. Through cluster analysis， the medicinal materials of 43 batches of Uncariae Ramulus Cum Uncis could be divided into 5 categories according to their different origins. Further principal component analysis revealed that the principal component comprehensive scores of Uncariae Ramulus Cum Uncis produced in Jiangxi and Hunan were relatively high， ranging from 0.264 to 2.904. The specific chromatogram could effectively distinguish among the different origins and their counterfeit products of Uncariae Ramulus Cum Uncis. CONCLUSIONS The established UPLC specific chromatogram can be used for quality control of Uncariae Ramulus Cum Uncis， and the study found that the quality of Uncariae Ramulus Cum Uncis from Jiangxi and Hunan provinces is relatively good.
