The role and mechanism of miRNA in STZ-induced mouse diabetic hepatopathy

Botao Jiang; Lirong Shu; Yun Wang; Shuqi LU; Lei Dong; Cheng Chen; Haiyun XU

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The role and mechanism of miRNA in STZ-induced mouse diabetic hepatopathy

VernacularTitle:miRNAs在小鼠糖尿病肝病模型中的作用及其机制
Author: Botao JIANG ^{1
,

2} ; Lirong SHU ¹ ; Yun WANG ¹ ; Shuqi LU ¹ ; Lei DONG ³ ; Cheng CHEN ⁴ ; Haiyun XU ⁴
Author Information

1. College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518055
2. The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an 710004
3. The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an 710004
4. Shantou University Medical College, Shantou 515041, China
Publication Type:Journal Article
Keywords: diabetic hepatopathy; Kunming mouse; bioinformatics; miRNA; mRNA
From: Journal of Xi'an Jiaotong University(Medical Sciences) 2021;42(5):666-673
CountryChina
Language:Chinese
Abstract: 【Objective】 To reveal possible mechanisms of miRNA in diabetic hepatopathy through bioinformatics method. 【Methods】 Subset data of miRNA and their matched mRNAs in the liver of STZ-induced diabetic mice and the normal liver tissues of congenial mice by detecting on microarrays were collected from GEO database； information from the database and bioinformatics analysis were applied to mine a batch of miRNAs in diabetic hepatopathy and targeted mRNAs regulated. Then qRT-PCR was used to verify the expressions of miRNAs in diabetic liver from 20 STZ-treated Kunming mice and 10 normal homologous mice. 【Results】 Via detection and analysis， miRNAs differentially expressed （including 96 up-regulated and 77 down-regulated） were significantly obtained. Groups of miRNAs and their effectors （mRNAs） that may be related to the pathological process of diabetic liver disease in mice were screened by GO and KEGG enrichment analyses， combined with relevant protein annotations in the databases and references. The expressions of miR-200a-3p， miR-200b-3p and miR-222-3p in the mice’s liver tissue detected by qRT-PCR were significantly down-regulated. In addition， the expressions of related effector genes CERS6， MYBL1， SCD2， SLCO1A4 and PLK2 were up-regulated， while the expressions of ACSS2， BCL6 and SLC10A2 were down-regulated. 【Conclusion】 The variation trend of those candidate miRNAs in mouse diabetic liver compared with that in control livers was consistent with that of the previous studies and prediction， which revealed their potential molecular regulation in this disease process.