Protective effect of exogenous H2 S on oxygen-glucose deprivation and reoxygenation-induced cell injury of SH-SY5Y cells
- VernacularTitle:外源性H
2 S对氧糖剥夺再灌注后SH-SY5Y细胞损伤的保护作用 - Author:
Changxue WU
1
;
Yanjun DONG
2
;
Yun HUANG
1
;
Zhiyu XIAO
1
;
Yi LI
1
;
Xiaolan QI
1
;
Zhizhong GUAN
1
;
Yan XIAO
1
Author Information
- Publication Type:Journal Article
- Keywords: vascular dementia; hydrogen sulfide; nuclear transcription factor-K B; apoptosis
- From: Journal of Xi'an Jiaotong University(Medical Sciences) 2021;42(5):659-665
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To explore the protective effects of exogenous hydrogen sulfide （H
2 S） on oxygen glucose deprivation and re-oxygenation （OGD/R）-induced SH-SY5Y cell injury. 【Methods】 OGD/R model was established in SH-SY5Y cells. Based on interferences， SH-SY5Y cells were divided into control group （no interference）， OGD/R model group， and treatment groups （OGD/R model treated with different concentrations of NaHS （H2 S donor）. The cells were cultured in low glucose medium without fetal bovine serum under 37 ℃， 93% N2 and hypoxia （2% O2 ） for 12 h， then in complete medium under normoxia （5% O2 ） for 24 h. CCK-8 was used to detect cell viability， and flow cytometry was used for assaying the level of cellular apoptosis. Western blot was used to detect the expression levels of endoplasmic reticulum stress-related proteins including glucose regulatory protein 78 （GRP78）， CCAAT/enhancer-binding homologous protein （CHOP）， NF-κB P65 and COX-2. 【Results】 The cell viability was lower in OGD/R model group than in the control group （P<0.05）. NaHS treatment at a concentration of 0-0.5 mmol/L increased cell viability in OGD/R model group in a dose-dependent manner （P<0.05）. In contrast， NaHS （C>0.5 mmol/L） decreased cell viability of OGD/R （P<0.05）. The apoptosis level of early stage in OGD/R model group was significantly higher than that in control group （P<0.05）. NaHS treatment at concentrations of 0.2 and 0.4 mmol/L lowered the apoptosis level of early stage in OGD/R model group （P<0.05）. Western blot showed that the protein expression levels of GRP78， CHOP， NF-κB p65， and COX-2 were significantly higher in OGD/R model group than those in control group （P<0.05）. When compared with OGD/R model group， the expression levels of GRP78， CHOP， NF-κBp65 and COX-2 were decreased by NaHS at concentrations of 0.2， 0.4 and 4 mmol/L （P<0.05）， but there was no statistically significant difference among the treatment groups （P>0.05）. 【Conclusion】 NaHS can rescue cell viability of OGD/R-induced cell injury. It may be achieved by inhibiting the activation of NF-κBp65 and COX-2 proteins induced by endoplasmic reticulum stress to reduce the level of cell apoptosis.
