Cinobufotalin regulating the proliferation and apoptosis of ovarian cancer cells SKOV3 through wee1 protein
- VernacularTitle:华蟾素通过wee1蛋白调控卵巢癌细胞SKOV3的增殖与凋亡
- Author:
Jing GUO
1
;
Yue GAO
2
Author Information
- Publication Type:Journal Article
- Keywords: wee1; cinobufotalin （CINO）; cell proliferation; cell apoptosis; ovarian cancer
- From: Journal of Xi'an Jiaotong University(Medical Sciences) 2021;42(6):857-861
- CountryChina
- Language:Chinese
- Abstract: 【Objective】 To observe whether cinobufotalin （CINO） inhibits the proliferation and improves apoptosis of ovarian cancer SKOV3 cells by inhibiting the expression of wee1. 【Methods】 PcDNA-wee1 was transfected into SKOV3 cells. SKOV3 cells that overexpressed with wee1 were constructed and identified by Real-time PCR and Western blotting. After that， 10 μg/mL CINO was used for intervention treatment and grouping. CCK8 assay was used to detect cell proliferation. Cell apoptosis was detected by flow cytometry after 48 h of treatment. Western blotting detected the expressions of Caspase-3， Bax and Bcl-2 in each group. 【Results】 Real-time PCR and Western blotting showed that the expression of wee1 was significantly increased in the pcDNA-wee1 group， while the expression of wee1 decreased in the CINO + pcDNA-wee1 group compared with the pcDNA-wee1 group. Compared with the pcDNA-wee1 group， the proliferation of SKOV3 cells in CINO + pcDNA-wee1group was inhibited （P<0.05）. Compared with pcDNA-wee1 group， the apoptosis rate of SKOV3 cells in CINO+pcDNA-wee1 group was increased （P<0.05）. Western blotting results showed that Bcl-2 protein expression in CINO+pcDNA-wee1 group was inhibited compared with that in pc-DNA-wee1 group， while Bax and Caspase-3 protein expressions were enhanced. 【Conclusion】 CINO can inhibit the proliferation and improve the apoptosis of ovarian cancer cells by reducing the expression of wee1.
