Effects of miR-29b-3p targeting PTEN on congenital heart disease
- VernacularTitle:miR-29b-3p靶向PTEN在先天性心脏病中的作用及其机制
- Author:
Ping LIU
1
;
Maiqiao YANG
1
;
Yetian YANG
1
;
Yiting WANG
1
;
Lili LIU
1
;
Yuanping MA
1
;
Fang WANG
1
;
Chuan HE
2
Author Information
- Publication Type:Journal Article
- Keywords: miR-29b-3p; congenital heart disease; targeted regulation
- From: Journal of Xi'an Jiaotong University(Medical Sciences) 2021;42(6):868-874
- CountryChina
- Language:Chinese
- Abstract: 【Objective】 To investigate the effects of miR-29b-3p on congenital heart disease and its mechanism. 【Methods】 The expression level of miR-29b-3p in serum from CHD patients and normal individuals， and in cells was detected by RT-qPCR. Dimethylsulfoxide （DMSO） was used to induce P19 cells to differentiate into cardiomyocytes. Western blot was used to measure the expression levels of cardiogenesis-associated genes， and phosphatase and tensin homolog （PTEN） level in cells. The proliferation and migration of cardiomyocytes were measured by CCK-8 and Transwell assay， respectively. Dual-luciferase gene reporter assay was used to verify the targeted relationship between miR-29b-3p and PTEN. 【Results】 Compared with that of normal individuals， the expression of miR-29b-3p in CHD patients was decreased. During differentiation， miR-29b-3p level was higher at late stage than that at early stage. Downregulated miR-29b-3p inhibited the differentiation of P19 cells into cardiomyocytes， and inhibited cell proliferation and migration. miR-29b-3p targeted PTEN. The increased PTEN level induced by miR-29b-3p knockdown inhibited the differentiation of P19 cells， and proliferation and migration of cardiomyocytes. 【Conclusion】 miR-29b-3p was downregulated in the serum of CHD patients. The downregulation of miR-29b-3p inhibited the differentiation of P19 cells， proliferation and migration of cardiomyocytes by targeting and regulating PTEN.
