Evaluation of IH-1000 for Automated ABO-Rh Typing and Irregular Antibody Screening.
- Author:
Youngchun PARK
1
;
Jinsook LIM
;
Younghuyn KO
;
Kyechul KWON
;
Sunhoe KOO
;
Jimyung KIM
Author Information
1. Department of Laboratory Medicine, College of Medicine, Chungnam National University, Daejeon, Korea. jmkim@cnuh.co.kr
- Publication Type:Original Article
- Keywords:
IH-1000;
ABO typing;
RhD typing;
Antibody screening;
Automation
- MeSH:
Agglutination;
Automation;
Blood Banks;
Isoantibodies;
Mass Screening;
Sensitivity and Specificity
- From:Korean Journal of Blood Transfusion
2012;23(2):127-135
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Despite modern advances in laboratory automated medicine, work-process in the blood bank is still handled manually. Several automated immunohematological instruments have been developed and are available in the market. The IH-1000 (Bio-Rad Laboratories, Hercules, CA, USA), a fully automated instrument for immunohematology, was recently introduced. In this study, we evaluated the performance of the IH-1000 for ABO/Rh typing and irregular antibody screening. METHODS: In October 2011, a total of 373 blood samples for ABO/Rh typing and 303 cases for unexpected antibody screening were collected. The IH-1000 was compared to the manual tube and slide methods for ABO/Rh typing and to the microcolumn agglutination method (DiaMed-ID system) for antibody screening. RESULTS: For ABO/Rh typing, concordance rate was 100%. For unexpected antibody screening, positive results for both column agglutination and IH-1000 were observed in 10 cases (four cases of anti-E and c, three of anti-E, one of anti-D, one of anti-M, and one of anti-Xg) and negative results for both were observed in 289 cases. The concordance rate between IH-1000 and column agglutination was 98.7%. Sensitivity and specificity were 90.9% and 99.3%, respectively. CONCLUSION: The automated IH-1000 showed good correlation with the manual tube and slide methods and the microcolumn agglutination method for ABO-RhD typing and irregular antibody screening. The IH-1000 can be used for routine pre-transfusion testing in the blood bank.
