Age Discrimination Based on Volatile Components of Arisaema Cum Bile
10.13422/j.cnki.syfjx.20231814
- VernacularTitle:基于胆南星挥发性成分的发酵年份识别
- Author:
Jia HE
1
;
Tiegui NAN
2
;
Tianrui LIU
3
;
Yuyang ZHAO
2
;
Ying LIU
4
;
Yan JIN
2
;
Yuan YUAN
2
Author Information
1. School of Traditional Chinese Medicine（TCM）， Guangdong Pharmaceutical University， Guangzhou 510006， China
2. State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs， National Resource Center for Chinese Materia Medica， China Academy of Chinese Medical Sciences， Beijing 100700， China
3. Institute of TCM Health Industry，China Academy of Chinese Medical Sciences，Nanchang，330115 China
4. Beijing Shuangqiao Yanjing Chinese Medicine Pieces Factory， Beijing 100024， China
- Publication Type:Journal Article
- Keywords:
Arisaema Cum Bile;
headspace solid-phase microextraction;
gas chromatography-mass spectrometry;
volatile components;
fermentation years
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2024;30(7):197-204
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo discriminate the age of Arisaema Cum Bile， the combination of headspace solid-phase microextraction （HS-SPME） with gas chromatography-mass spectrometry （GC-MS） was applied to explore the differences of volatile components of unfermented， 1-year fermented， 2-year fermented， and 3-year fermented Arisaema Cum Bile. MethodSamples with different fermentation durations were collected and HS-SPME-GC-MS technology was employed to detect the volatile components of each sample. The relative contents of detected volatile components were processed and analyzed by chemometrics methods such as principal component analysis （PCA）， hierarchical cluster analysis （HCA）， and partial least squares discriminant analysis （PLS-DA）. ResultThe results showed that 145 volatile components were identified. Among these volatile components， the relative contents of heterocyclic， alcohols， aldehydes and aromatics were high. PCA， HCA， and PLS-DA can effectively separate Arisaema Cum Bile with four different ages. Based on variable importance in projection （VIP） value > 1， 73 markers of differential volatile components were identified. The content of 2，6，11-trimethyldodecane and m-xylene in unfermented samples was the highest， and the content difference between them and those in fermented samples was significant （P<0.05）. 2，3-butanediol was detected only in 1-year samples， octane was detected only in 2-year samples， and ethyl heptanoate was detected only in 3-year samples. These components can be used as odor markers for Arisaema Cum Bile with different fermentation years. ConclusionThe identification method of volatile components of Arisaema Cum Bile was established by HS-SPME-GC-MS technology， which can realize the rapid identification of unfermented， 1-year fermented， 2-year fermented， and 3-year fermented samples， and provide a scientific basis for the standardization of processing technology and quality standards of Arisaema Cum Bile.
