Comparison of the efficiency of different etiological assays for detection of Schistosoma japonicum infections in wild mice
10.16250/j.32.1374.2023076
- VernacularTitle:不同病原学方法检测野鼠日本血吸虫感染效果比较
- Author:
Xiaojuan XU
1
;
Xuefeng CHEN
2
;
Fan WU
3
;
Chenyang WU
4
;
Ting LIU
1
;
Bo DAI
1
;
Tianping WANG
1
;
Shiqing ZHANG
1
Author Information
1. Anhui Provincial Institute of Schistosomiasis Control, Hefei, Anhui 230601, China
2. Shitai County Center for Disease Control and Prevention, Anhui Province, China
3. Wuhu Municipal Station for Endemic Disease Control, Anhui Province, China
4. Guichi District Station of Schistosomiasis Control, Chizhou City, Anhui Province, China
- Publication Type:Journal Article
- Keywords:
Schistosoma japonicum;
Wild mouse;
Etiological assay;
Detection efficiency
- From:
Chinese Journal of Schistosomiasis Control
2023;35(6):573-582
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the efficiency of multiple etiological techniques for detection of Schistosoma japonicum infections in wild mice, so as to provide technical supports to assessment of schistosomiasis transmission risk. Methods Wild mice were captured with baited traps at night in Oncomelania hupensis snail-infested settings in schistosomiasis-endemic foci of Anhui Province from October to November, 2022. S. japonicum infections were detected in wild mice using microscopy of mouse liver tissues, microscopy of mouse mesenteric tissues, microscopy of mouse liver tissue homogenates, miracidial hatching test of mouse liver tissue homogenates, Kato-Katz technique and miracidial hatching test of mouse stool samples alone and in combinations. Identification of S. japonicum eggs or miracidia by any of these six assays was defined as an infection. The sensitivity of six assays alone or in combinations was compared for detection of S. japonicum infections in wild mice. Results A total of 1 703 wild mice were captured, with 366 wild mice detected positive for S. japonicum (21.49%). There were significant differences in the prevalence of S. japonicum infections in wild mice by six assays (Q = 529.33, P < 0.001) and in the sensitivity of six assays for detection of S. japonicum infections in wild mice (χ2 = 527.78, P < 0.001). In addition, the combination of microscopy of mouse liver tissues and mesenteric tissues, combination of microscopy of mouse liver tissues and liver tissue homogenates and combination of microscopy of mouse liver tissues, microscopy of mesenteric tissues, microscopy of liver tissue homogenates and Kato-Katz technique showed 86.61%, 87.16% and 97.27% sensitivities for detection of S. japonicum infections in wild mice, respectively. Conclusions Diverse etiological assays show various efficiencies for detection of S. japonicum infections in wild mice. Combination of microscopy of mouse liver tissues and microscopy of mesenteric tissues, and combination of microscopy of mouse liver tissues and microscopy of liver tissue homogenates are potential approaches for field detection of S. japonicum infections in wild mice.
