Establishment of a method for detecting propofol concentration in plasma and its application in patients with lymphedema
- VernacularTitle:丙泊酚血药浓度检测方法建立及在淋巴水肿患者中的应用
- Author:
Jingfeng LI
1
;
Zhengyuan SHI
1
;
Mengjie ZHANG
2
;
Lei GUAN
2
;
Dechun JIANG
1
Author Information
1. Dept. of Pharmacy，Beijing Shijitan Hospital Affiliated to Capital Medical Hospital，Beijing 100038，China
2. Dept. of Anesthesiology，Beijing Shijitan Hospital Affiliated to Capital Medical Hospital，Beijing 100038，China
- Publication Type:Journal Article
- Keywords:
UPLC-MS/MS;
propofol;
lymphedema;
plasma concentration
- From:
China Pharmacy
2024;35(4):476-480
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To establish a method for the determination of propofol concentration in human plasma and apply it in patients with lymphedema. METHODS The concentration of propofol was determined by UPLC-MS/MS after protein precipitation of plasma samples using thymol as internal standard. The sample was eluted on a Kinetex C18 column with a mobile phase consisting of acetonitrile （A）-water （B） for gradient elution at the flow rate of 200 μL/min. The sample size was 5 μL， and the column temperature was set at 40 ℃. The sample chamber temperature was 15 ℃. Using multi-reaction monitoring mode， the ion pairs for quantitative analysis were m/z 177.0→161.2 （propofol） and m/z 149.0→133.1 （internal standard）， respectively. The above method was used to determine the plasma concentration of propofol in 6 patients with lymphedema. RESULTS The linear range of propofol was 50-5 000 ng/mL （r＝0.995 0）. RSDs of within- and between-batch precision were not more than 8.08%； no endogenous interference， carryover effect， or dilution effect was observed in blank plasma. The extraction recovery ranged from 89.80% to 93.73%， and matrix effects were within the range of 97.93%-101.73%. RSDs of the stability test were all lower than 3.27%. During intraoperative TCI 2-30 min， the plasma concentration of propofol in 6 patients was maintained in the range of 1 865.3-6 056.2 ng/mL， and the propofol was almost excreted within 4-8 h after operation. CONCLUSIONS The established UPLC-MS/MS method in this study can achieve the determination of propofol and a simple and fast sample pretreatment process without derivatization； it is proved to be suitable for the concentration monitoring of propofol in plasma samples of patients with lymphedema.
