A non-toxic recombinant protein rSUMO-CPBm4 as a potential vaccine candidate  against Clostridium perfringens type C beta enterotoxemia

Y Gao; JG Du; C Jirapattharasate; E Galon; SW Ji; ZG Ran; YQ Xia

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A non-toxic recombinant protein rSUMO-CPBm4 as a potential vaccine candidate against Clostridium perfringens type C beta enterotoxemia

Author: Gao, Y. ^{1
,

2} ; Du, J.G. ³ ; Jirapattharasate, C. ⁴ ; Galon, E. ⁵ ; Ji, S.W. ⁵ ; Ran, Z.G. ² ; Xia, Y.Q. ¹
Author Information

1. The Key Sericultural Laboratory of Agricultural Ministry, College of Biotechnology, Southwest University, Chongqing, 400715, PR China&
2. Chongqing Auleon Biological Co., Ltd., Rongchang, Chongqing, 402460, PR China
3. Department of Bacterial Biologics, China Institute of Veterinary Drug Control, No. 8 Zhongguancun South Street, Beijing 100-081, China
4. Department of Pre-Clinic and Applied Animal Science, Faculty of Veterinary Science, Mahidol University, 999 Phutthamonthon Sai4, Salaya, Phutthamonthon, Nakhon Pathom, Thailand, 73170
5. National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan
Publication Type:Journal Article
Keywords: Beta toxin; Clostridium perfringens type C; detoxified; solubility; substitution.
From:Tropical Biomedicine 2023;40(No.4):400-405
CountryMalaysia
Language:English
Abstract: Beta toxin (CPB) is a lethal toxin and plays a key role in enterotoxemia of ruminants caused by Clostridium perfringens type C strain. The existing vaccines based on crude CPB need time-consuming detoxification and difficult quality control steps. In this study, we synthesized the rCPBm4 of C. perfringens type C strain and small ubiquitin-like modifier (SUMO)-tag CPBm4 (rSUMO-CPBm4) by introducing four amino acid substitutions: R212E, Y266A, L268G, and W275A. Compared with rCPBm4, rSUMO-CPBm4 was expressed with higher solubility in Escherichia coli BL21 (DE3). Neither rCPBm4 nor rSUMO-CPBm4 was lethal to mice. Although rCPBm4 and rSUMO-CPBm4 were reactogenic with polyclonal antibodies against crude CPB, rabbits vaccinated with rSUMO-CPBm4 developed significant levels of toxin-neutralizing antibody (TNA) titers that conferred protection against crude toxin challenge. These data suggest that genetically detoxified rSUMO-CPBm4 is a promising subunit vaccine candidate for C. perfringens type C beta enterotoxemia.
Full text:8.2023my1435.pdf