Evolution of chemistry and selection technology for DNA-encoded library.
10.1016/j.apsb.2023.10.001
- Author:
Peixiang MA
1
;
Shuning ZHANG
1
;
Qianping HUANG
2
;
Yuang GU
2
;
Zhi ZHOU
3
;
Wei HOU
4
;
Wei YI
3
;
Hongtao XU
2
Author Information
1. Shanghai Key Laboratory of Orthopedic Implants, Department of Orthopedics, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai 200025, China.
2. Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai 201210, China.
3. Guangzhou Municipal and Guangdong Provincial Key Laboratory of Molecular Target & Clinical Pharmacology, The NMPA and State Key Laboratory of Respiratory Disease, Guangzhou Medical University, Guangzhou 511436, China.
4. College of Pharmaceutical Science and Institute of Drug Development & Chemical Biology, Zhejiang University of Technology, Hangzhou 310014, China.
- Publication Type:Review
- Keywords:
Chemical central dogma;
DNA-compatible synthesis;
DNA-encoded library;
Drug discovery;
High-throughput selection;
In vitro evolution
- From:
Acta Pharmaceutica Sinica B
2024;14(2):492-516
- CountryChina
- Language:English
-
Abstract:
DNA-encoded chemical library (DEL) links the power of amplifiable genetics and the non-self-replicating chemical phenotypes, generating a diverse chemical world. In analogy with the biological world, the DEL world can evolve by using a chemical central dogma, wherein DNA replicates using the PCR reactions to amplify the genetic codes, DNA sequencing transcripts the genetic information, and DNA-compatible synthesis translates into chemical phenotypes. Importantly, DNA-compatible synthesis is the key to expanding the DEL chemical space. Besides, the evolution-driven selection system pushes the chemicals to evolve under the selective pressure, i.e., desired selection strategies. In this perspective, we summarized recent advances in expanding DEL synthetic toolbox and panning strategies, which will shed light on the drug discovery harnessing in vitro evolution of chemicals via DEL.
