Purpurolide C-based microneedle promotes macrophage-mediated diabetic wound healing via inhibiting TLR4-MD2 dimerization and MYD88 phosphorylation.
10.1016/j.apsb.2023.05.032
- Author:
Yitong LIU
1
;
Guiyang XIA
2
;
Yingyi CHEN
1
;
Huan XIA
2
;
Junji XU
1
;
Lijia GUO
3
;
Sheng LIN
2
;
Yi LIU
1
Author Information
1. Laboratory of Tissue Regeneration and Immunology and Department of Periodontics, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, School of Stomatology, Capital Medical University, Beijing 100050, China.
2. Key Laboratory of Chinese Internal Medicine of Ministry of Education and Beijing, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China.
3. Department of Orthodontics, School of Stomatology, Capital Medical University, Beijing 100050, China.
- Publication Type:Journal Article
- Keywords:
Diabetes;
Drug delivery;
Inflammation;
Liposome and GelMA-based microneedle patches;
Macrophage polarization;
Molecular mechanism;
Purpurolide C;
Wound healing
- From:
Acta Pharmaceutica Sinica B
2023;13(12):5060-5073
- CountryChina
- Language:English
-
Abstract:
Delayed wound healing in diabetes is a global challenge, and the development of related drugs is a clinical problem to be solved. In this study, purpurolide C (PC), a small-molecule secondary metabolite of the endophytic fungus Penicillium purpurogenum, was found to promote diabetic wound healing. To investigate the key regulation targets of PC, in vitro RNA-seq, molecular docking calculations, TLR4-MD2 dimerization SDS-PAGE detection, and surface plasmon resonance (SPR) were performed, indicating that PC inhibited inflammatory macrophage activation by inhibiting both TLR4-MD2 dimerization and MYD88 phosphorylation. Tlr4 knockout in vivo attenuated the promotion effect of PC on wound healing. Furthermore, a delivery system consisting of macrophage liposome and GelMA-based microneedle patches combined with PC (PC@MLIP MN) was developed, which overcame the poor water solubility and weak skin permeability of PC, so that successfully punctured the skin and delivered PC to local tissues, and accurately regulated macrophage polarization in diabetic wound management. Overall, PC is an anti-inflammatory small molecule compound with a well-defined structure and dual-target regulation, and the PC@MLIP MN is a promising novel biomaterial for the management of diabetic wound.
