Altered expression of 15-hydroxyprostaglandin dehydrogenase in chronic rhinosinusitis with nasal polyps.
10.13201/j.issn.2096-7993.2023.11.007
- Author:
Shan CHEN
1
;
Jingcai CHEN
1
;
Jianjun CHEN
1
;
Yanjun WANG
1
Author Information
1. Department of Otolaryngology Head and Neck Surgery，Affiliated Union Hospital of Tongji Medical College，Huazhong University of Science and Technology，Wuhan，430022，China.
- Publication Type:Journal Article
- Keywords:
15-hydroxyprostaglandin dehydrogenase;
chronic rhinosinusitis with nasal polyps;
high mobility group box-1
- MeSH:
Humans;
Antibodies, Neutralizing/metabolism*;
Chronic Disease;
HMGB1 Protein/metabolism*;
Mitogen-Activated Protein Kinase Kinases/metabolism*;
Nasal Mucosa/metabolism*;
Nasal Polyps/metabolism*;
Rhinitis
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2023;37(11):891-896
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression level and regulatory mechanism of 15-hydroxyprostaglandin dehydrogenase（HPGD） in chronic rhinosinusitis with nasal polyps（CRSwNP）. Methods:The expression pattern and level of HPGD in CRSwNP and control was observed using immunofluorescence, and western blot was used for analysis of HPGD expression in nasal polyp tissues. The effect of recombinant human high mobility group box-1（HMGB1） on HPGD expression in primary human nasal epithelial cells was observed, and the potential blocking effect of RAGE neutralizing antibody on HMGB1-induced HPGD expression was investigated. Results:The expression of HPGD was elevated in CRSwNP patients compared to the control, while the protein mainly localized at CD68-positive cells and epithelial cells. Recombinant human HMGB1 stimulated an increase in HPGD expression in primary human nasal mucosal epithelial cells at a time-dependent manner. Additionally, increased phosphorylation levels of MEK and elevated RAGE expression were also observed at 12 hours, but decreased at 24 hours after the incubation of HMGB1. The increase in the expression of HPGD induced by HMGB1 in primary human nasal epithelial cells was partly inhibited with RAGE neutralizing antibody. Conclusion:Elevated HPGD expression is observed in CRSwNP, predominantly in macrophages and epithelial cells. HMGB1 regulates HPGD expression through the RAGE-MEK signaling pathway, potentially providing a new target for future regulation of PGE2levels in CRSwNP.
