Scutellarin alleviates liver injury in type 2 diabetic mellitus by suppressing hepatocyte apoptosis in vitro and in vivo.
10.1016/j.chmed.2023.03.007
- Author:
Xiaoming FAN
1
;
Yiyu WANG
2
;
Xifan LI
1
;
Taiqing ZHONG
2
;
Chunlan CHENG
2
;
Yunfei ZHANG
3
Author Information
1. Department of Human Anatomy, School of Basic Medicine, Guilin Medical University, Guilin 541004, China.
2. Department of Laboratory, The Affiliated Women's and Children's Hospital of Chengdu Medical College, Chengdu 610045, China.
3. Department of Physiology, School of Basic Medicine, Guilin Medical University, Guilin 541004, China.
- Publication Type:Journal Article
- Keywords:
apoptosis;
hepatocyte cells;
liver failure;
scutellarin;
type 2 diabetic mellitus
- From:
Chinese Herbal Medicines
2023;15(4):542-548
- CountryChina
- Language:English
-
Abstract:
OBJECTIVE:Scutellarin is a primary active composition come from Erigeron breviscapus. It is well known that scutellarin has anti-inflammatory and antioxidant physiological functions. In this study, we detected the effects of scutellarin on hepatocyte cell apoptosis in type 2 diabetes mellitus (T2DM) rats.
METHODS:Sprague Dawley (SD) (6-8 weeks, 160-180 g) rats were randomly divided into six groups: control, model, scutellarin low-dose, medium-dose, high-dose treatment, and rosiglitazone positive groups; with 10 SD rats in each group (n = 10). The changes of biochemical factors in serum were detected by automatic biochemical instrument, the pathological changes of liver tissue were detected by hematoxylin and eosin (HE) staining, the apoptosis of liver tissue and cells was detected by tissue staining and flow analyzer, and the expression of apoptosis-related factors were determined by qPCR, Western blot and immunohistochemistry in liver tissues or cells.
RESULTS:The results showed that scutellarin decreased the levels of fasting blood glucose, total cholesterol, triglyceride, and low-density lipoprotein and increased the levels of high-density lipoprotein. Meanwhile, scutellarin decreased the levels of alanine transaminase (ALT) and aspartate transaminase (AST) and improved liver function. In addition, scutellarin suppressed the secretion of interleukin-1 (IL-1), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) and reduced hepatocyte apoptosis. Furthermore, scutellarin inhibited the expression of cleaved Caspase-3, Bax, and cytochrome C (Cyt-C) and promoted the expression of Bcl-2.
CONCLUSION:Scutellarin can inhibit the apoptotic pathway, thereby relieving T2DM.
