Whole-brain Optical Imaging: A Powerful Tool for Precise Brain Mapping at the Mesoscopic Level.
10.1007/s12264-023-01112-y
- Author:
Tao JIANG
1
;
Hui GONG
1
;
Jing YUAN
2
Author Information
1. Huazhong University of Science and Technology-Suzhou Institute for Brainsmatics, Jiangsu Industrial Technology Research Institute, Suzhou, 215123, China.
2. Huazhong University of Science and Technology-Suzhou Institute for Brainsmatics, Jiangsu Industrial Technology Research Institute, Suzhou, 215123, China. yuanj@hust.edu.cn.
- Publication Type:Review
- Keywords:
Brain connectome;
Micrometer resolution;
Neural circuits;
Neuron;
Optical sectioning;
Whole-brain optical imaging
- MeSH:
Animals;
Brain/physiology*;
Brain Mapping/methods*;
Neurons/physiology*;
Optical Imaging/methods*;
Mammals
- From:
Neuroscience Bulletin
2023;39(12):1840-1858
- CountryChina
- Language:English
-
Abstract:
The mammalian brain is a highly complex network that consists of millions to billions of densely-interconnected neurons. Precise dissection of neural circuits at the mesoscopic level can provide important structural information for understanding the brain. Optical approaches can achieve submicron lateral resolution and achieve "optical sectioning" by a variety of means, which has the natural advantage of allowing the observation of neural circuits at the mesoscopic level. Automated whole-brain optical imaging methods based on tissue clearing or histological sectioning surpass the limitation of optical imaging depth in biological tissues and can provide delicate structural information in a large volume of tissues. Combined with various fluorescent labeling techniques, whole-brain optical imaging methods have shown great potential in the brain-wide quantitative profiling of cells, circuits, and blood vessels. In this review, we summarize the principles and implementations of various whole-brain optical imaging methods and provide some concepts regarding their future development.
