The Combined Effects of Ginkgo Biloba Extracts and Aspirin on Viability of SK-N-MC, Neuroblastoma Cell Line in Hypoxia and Reperfusion Condition.
10.3340/jkns.2011.49.1.13
- Author:
Sung Hwan MOON
1
;
Yong Jik LEE
;
Soo Yong PARK
;
Kwan Young SONG
;
Min Ho KONG
;
Jung Hee KIM
Author Information
1. Department of Neurosurgery, Seoul Medical Center, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Ginkgo biloba extract;
Aspirin;
GAP43;
MAP2;
Bcl2;
p53
- MeSH:
Anoxia;
Aspirin;
B-Lymphocytes;
Cell Count;
Cell Line;
Cell Survival;
Ginkgo biloba;
Ginkgolides;
Humans;
Incubators;
Lactones;
Microtubule-Associated Proteins;
Neuroblastoma;
Regeneration;
Reperfusion;
Reverse Transcriptase Polymerase Chain Reaction;
RNA, Messenger
- From:Journal of Korean Neurosurgical Society
2011;49(1):13-19
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVE: The purpose of this study is to investigate the combined effects of ginkgo biloba extract, ginkgolide A and B and aspirin on SK-N-MC, human neuroblastoma cell viability and mRNA expression of growth associated protein43 (GAP43), Microtubule-associated protein 2 (MAP2), B-cell lymphoma2 (Bcl2) and protein53 (p53) gene in hypoxia and reperfusion condition. METHODS: SK-N-MC cells were cultured with Dulbecco's Modified Eagle's Medium (DMEM) media in 37degrees C, 5% CO2 incubator. The cells were cultured for 8 hours in non-glucose media and hypoxic condition and for 12 hours in normal media and O2 concentration. Cell survival rate was measured with Cell Counting Kit-8 (CCK-8) reagent assay. Reverse transcriptase polymerase chain reaction (RT-PCR) was used to estimate mRNA levels of GAP43, MAP2, Bcl2, and p53 genes. RESULTS: The ginkgolide A and B increased viable cell number decreased in hypoxic and reperfused condition. The co-treatment of ginkgolide B with aspirin also increased the number of viable cells, however, there was no additive effect. Although there was no increase of mRNA expression of GAP43, MAP2, and Bcl2 in SK-N-MC cells with individual treatment of ginkgolide A, B or aspirin in hypoxic and reperfused condition, the co-treatment of ginkgolide A or B with aspirin significantly increased GAP43 and Bcl2 mRNA levels. In MAP2, only the co-treatment of ginkgolide A and aspirin showed increasing effect. The mRNA expression of p53 had no change in all treating conditions. CONCLUSION: This study suggests that the combined treatments of Ginkgo biloba extracts and aspirin increase the regeneration of neuroblastoma cells injured by hypoxia and reperfusion.
