A Novel Retrograde AAV Variant for Functional Manipulation of Cortical Projection Neurons in Mice and Monkeys.
10.1007/s12264-023-01091-0
- Author:
Yefei CHEN
1
;
Jingyi WANG
2
;
Jing LIU
1
;
Jianbang LIN
2
;
Yunping LIN
2
;
Jinyao NIE
2
;
Qi YUE
2
;
Chunshan DENG
2
;
Xiaofei QI
3
;
Yuantao LI
1
;
Ji DAI
4
;
Zhonghua LU
5
Author Information
1. Department of Anesthesiology, Shenzhen Maternity and Child Healthcare Hospital, The First School of Clinical Medicine, Southern Medical University, Shenzhen, 518027, China.
2. Shenzhen Technological Research Center for Primate Translational Medicine, Shenzhen Key Laboratory for Molecular Biology of Neural Development, Shenzhen-Hong Kong Institute of Brain Science, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, China.
3. Department of Anesthesiology, Shenzhen Maternity and Child Healthcare Hospital, The First School of Clinical Medicine, Southern Medical University, Shenzhen, 518027, China. xiaofeiqi@smu.edu.cn.
4. Shenzhen Technological Research Center for Primate Translational Medicine, Shenzhen Key Laboratory for Molecular Biology of Neural Development, Shenzhen-Hong Kong Institute of Brain Science, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, China. ji.dai@siat.ac.cn.
5. Shenzhen Technological Research Center for Primate Translational Medicine, Shenzhen Key Laboratory for Molecular Biology of Neural Development, Shenzhen-Hong Kong Institute of Brain Science, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, China. zh.lu@siat.ac.cn.
- Publication Type:Journal Article
- Keywords:
Capsid variant;
Cortical projection neuron;
Monkey;
Optogenetics;
Retrograde AAV
- MeSH:
Animals;
Haplorhini;
Axons;
Motor Neurons;
Interneurons;
Macaca;
Dependovirus/genetics*;
Genetic Vectors
- From:
Neuroscience Bulletin
2024;40(1):90-102
- CountryChina
- Language:English
-
Abstract:
Retrograde adeno-associated viruses (AAVs) are capable of infecting the axons of projection neurons and serve as a powerful tool for the anatomical and functional characterization of neural networks. However, few retrograde AAV capsids have been shown to offer access to cortical projection neurons across different species and enable the manipulation of neural function in non-human primates (NHPs). Here, we report the development of a novel retrograde AAV capsid, AAV-DJ8R, which efficiently labeled cortical projection neurons after local administration into the striatum of mice and macaques. In addition, intrastriatally injected AAV-DJ8R mediated opsin expression in the mouse motor cortex and induced robust behavioral alterations. Moreover, AAV-DJ8R markedly increased motor cortical neuron firing upon optogenetic light stimulation after viral delivery into the macaque putamen. These data demonstrate the usefulness of AAV-DJ8R as an efficient retrograde tracer for cortical projection neurons in rodents and NHPs and indicate its suitability for use in conducting functional interrogations.
