Induction of Anxiety-Like Phenotypes by Knockdown of Cannabinoid Type-1 Receptors in the Amygdala of Marmosets.
10.1007/s12264-023-01081-2
- Author:
Lin ZHU
1
;
Di ZHENG
1
;
Rui LI
1
;
Chen-Jie SHEN
2
;
Ruolan CAI
1
;
Chenfei LYU
1
;
Binliang TANG
3
;
Hao SUN
1
;
Xiaohui WANG
1
;
Yu DING
1
;
Bin XU
3
;
Guoqiang JIA
1
;
Xinjian LI
1
;
Lixia GAO
4
;
Xiao-Ming LI
5
Author Information
1. Department of Neurobiology and Department of Neurology of Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310058, China.
2. NHC and CAMS Key Laboratory of Medical Neurobiology, MOE Frontier Science Center for Brain Research and Brain-Machine Integration, School of Brain Science and Brian Medicine, Zhejiang University, Hangzhou, 310058, China.
3. Interdisciplinary Institute of Neuroscience and Technology, Zhejiang University School of Medicine, Hangzhou, 310029, China.
4. Department of Neurobiology and Department of Neurology of Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310058, China. lxgao10@zju.edu.cn.
5. Department of Neurobiology and Department of Neurology of Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310058, China. lixm@zju.edu.cn.
- Publication Type:Journal Article
- Keywords:
Amygdala;
Anxiety;
CRISPR/Cas9;
Cannabinoid type-1 receptor;
Marmoset
- MeSH:
Animals;
Callithrix;
Receptors, Cannabinoid;
Anxiety;
Amygdala;
Cannabinoids;
Phenotype
- From:
Neuroscience Bulletin
2023;39(11):1669-1682
- CountryChina
- Language:English
-
Abstract:
The amygdala is an important hub for regulating emotions and is involved in the pathophysiology of many mental diseases, such as depression and anxiety. Meanwhile, the endocannabinoid system plays a crucial role in regulating emotions and mainly functions through the cannabinoid type-1 receptor (CB1R), which is strongly expressed in the amygdala of non-human primates (NHPs). However, it remains largely unknown how the CB1Rs in the amygdala of NHPs regulate mental diseases. Here, we investigated the role of CB1R by knocking down the cannabinoid receptor 1 (CNR1) gene encoding CB1R in the amygdala of adult marmosets through regional delivery of AAV-SaCas9-gRNA. We found that CB1R knockdown in the amygdala induced anxiety-like behaviors, including disrupted night sleep, agitated psychomotor activity in new environments, and reduced social desire. Moreover, marmosets with CB1R-knockdown had up-regulated plasma cortisol levels. These results indicate that the knockdown of CB1Rs in the amygdala induces anxiety-like behaviors in marmosets, and this may be the mechanism underlying the regulation of anxiety by CB1Rs in the amygdala of NHPs.
