Fgf8P2A-3×GFP/+: A New Genetic Mouse Model for Specifically Labeling and Sorting Cochlear Inner Hair Cells.
10.1007/s12264-023-01069-y
- Author:
Yi PAN
1
;
Shuting LI
2
;
Shunji HE
2
;
Guangqin WANG
2
;
Chao LI
2
;
Zhiyong LIU
3
;
Mingliang XIANG
4
Author Information
1. Department of Otolaryngology and Head and Neck Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, China.
2. Institute of Neuroscience, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai, 200031, China.
3. Institute of Neuroscience, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai, 200031, China. Zhiyongliu@ion.ac.cn.
4. Department of Otolaryngology and Head and Neck Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, China. xml12128@rjh.com.cn.
- Publication Type:Journal Article
- Keywords:
Cochlea;
Fgf8;
Inner Ear;
Inner Hair Cell
- MeSH:
Animals;
Mice;
Hair Cells, Auditory, Inner;
Cochlea/metabolism*;
Hair Cells, Auditory, Outer/metabolism*;
Disease Models, Animal;
Fibroblast Growth Factor 8/metabolism*
- From:
Neuroscience Bulletin
2023;39(12):1762-1774
- CountryChina
- Language:English
-
Abstract:
The cochlear auditory epithelium contains two types of sound receptors, inner hair cells (IHCs) and outer hair cells (OHCs). Mouse models for labelling juvenile and adult IHCs or OHCs exist; however, labelling for embryonic and perinatal IHCs or OHCs are lacking. Here, we generated a new knock-in Fgf8P2A-3×GFP/+ (Fgf8GFP/+) strain, in which the expression of a series of three GFP fragments is controlled by endogenous Fgf8 cis-regulatory elements. After confirming that GFP expression accurately reflects the expression of Fgf8, we successfully obtained both embryonic and neonatal IHCs with high purity, highlighting the power of Fgf8GFP/+. Furthermore, our fate-mapping analysis revealed, unexpectedly, that IHCs are also derived from inner ear progenitors expressing Insm1, which is currently regarded as an OHC marker. Thus, besides serving as a highly favorable tool for sorting early IHCs, Fgf8GFP/+ will facilitate the isolation of pure early OHCs by excluding IHCs from the entire hair cell pool.
