miRNA-128-3p inhibits malignant behavior of glioma cells by downregulating KLHDC8A expression.
10.12122/j.issn.1673-4254.2023.09.01
- VernacularTitle:miRNA-128-3p通过下调KLHDC8A的表达抑制胶质瘤细胞的恶性生物学行为
- Author:
Zhengtao YU
1
;
Jiameng LI
1
;
Junwen JIANG
1
;
You LI
1
;
Long LIN
1
;
Ying XIA
1
;
Lei WANG
2
Author Information
1. Department of Neurosurgery, Affiliated Haikou Hospital of Xiangya School of Central South University, Haikou 570208, China.
2. Department of Neurosurgery, Hunan Cancer Hospital and Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha 410006, China.
- Publication Type:Journal Article
- Keywords:
KLHDC8A;
biomarker;
glioma;
miRNA-128-3p;
migration;
proliferation
- MeSH:
Humans;
Apoptosis;
Cell Line, Tumor;
Cell Movement;
Cell Proliferation;
Gene Expression Regulation, Neoplastic;
Glioma/pathology*;
MicroRNAs/metabolism*;
Up-Regulation
- From:
Journal of Southern Medical University
2023;43(9):1447-1459
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To determine whether miRNA-128-3p regulates malignant biological behavior of glioma cells by targeting KLHDC8A.
METHODS:Dual-luciferase reporter assays, qRT-PCR and Western blotting were used to verify the targeting of miRNA-128-3p to KLHDC8A. Edu assay, flow cytometry, Transwell assay and would healing assay were used to determine the effects of changes in miRNA-128-3p and KLHDC8A expression levels on malignant behavior of glioma cells. Rescue experiment was carried out to verify that miRNA-128-3p regulated glioma cell proliferation, apoptosis, invasion and migration by targeting KLHDC8A.
RESULTS:The expression level of KLHDC8A was significantly increased in high-grade glioma tissue and was closely related to a poor survival outcome of the patients. Overexpression of KLHDC8A promoted glioma cell proliferation, migration and invasion, and miRNA-128-3p overexpression inhibited proliferative and metastatic capacities of glioma cells. Mechanistically, KLHDC8A expression was directly modulated by miRNA-128-3p, which, by targeting KLHDC8A, inhibited malignant behavior of glioma cells.
CONCLUSION:Upregulation of miRNA-128-3p inhibits uncontrolled growth of glioma cells by negatively regulating KLHDC8A expression and its downstream effectors, suggesting that the miRNA-128-3p-KLHDC8A axis may serve as a potential prognostic indicator and a therapeutic target for developing new strategies for glioma treatment.
