Bioceramic scaffolds with two-step internal/external modification of copper-containing polydopamine enhance antibacterial and alveolar bone regeneration capability.

Xiaojian Jiang; Lihong Lei; Weilian Sun; Yingming Wei; Jiayin Han; Shuaiqi Zhong; Xianyan Yang; Zhongru Gou; Lili Chen

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Bioceramic scaffolds with two-step internal/external modification of copper-containing polydopamine enhance antibacterial and alveolar bone regeneration capability.

Author: Xiaojian JIANG ¹ ; Lihong LEI ¹ ; Weilian SUN ¹ ; Yingming WEI ¹ ; Jiayin HAN ¹ ; Shuaiqi ZHONG ¹ ; Xianyan YANG ² ; Zhongru GOU ³ ; Lili CHEN ⁴
Author Information

1. Department of Oral Medicine, the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310009, China.
2. Bio-nanomaterials and Regenerative Medicine Research Division, Zhejiang‒California International Nanosystem Institute, Zhejiang University, Hangzhou 310058, China.
3. Bio-nanomaterials and Regenerative Medicine Research Division, Zhejiang‒California International Nanosystem Institute, Zhejiang University, Hangzhou 310058, China. chenlili_1030@zju.edu.cn, zhrgou@zju.edu.cn.
4. Department of Oral Medicine, the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310009, China. chenlili_1030@zju.edu.cn.
Publication Type:Journal Article
Keywords: Angiogenesis; Antibacterial property; Bioceramic scaffold; Bone regeneration; Copper-containing polydopamine; Modification
MeSH: Animals; Rabbits; Copper/pharmacology*; Tissue Scaffolds/chemistry*; Bone Regeneration; Anti-Bacterial Agents/pharmacology*; Osteogenesis; Calcium; Ions/pharmacology*
From: Journal of Zhejiang University. Science. B 2024;25(1):65-82
CountryChina
Language:English
Abstract: Magnesium-doped calcium silicate (CS) bioceramic scaffolds have unique advantages in mandibular defect repair; however, they lack antibacterial properties to cope with the complex oral microbiome. Herein, for the first time, the CS scaffold was functionally modified with a novel copper-containing polydopamine (PDA(Cu^2+‍)) rapid deposition method, to construct internally modified (*P), externally modified (@PDA), and dually modified (*P@PDA) scaffolds. The morphology, degradation behavior, and mechanical properties of the obtained scaffolds were evaluated in vitro. The results showed that the CS*P@PDA had a unique micro-/nano-structural surface and appreciable mechanical resistance. During the prolonged immersion stage, the release of copper ions from the CS*P@PDA scaffolds was rapid in the early stage and exhibited long-term sustained release. The in vitro evaluation revealed that the release behavior of copper ions ascribed an excellent antibacterial effect to the CS*P@PDA, while the scaffolds retained good cytocompatibility with improved osteogenesis and angiogenesis effects. Finally, the PDA(Cu²⁺)-modified scaffolds showed effective early bone regeneration in a critical-size rabbit mandibular defect model. Overall, it was indicated that considerable antibacterial property along with the enhancement of alveolar bone regeneration can be imparted to the scaffold by the two-step PDA(Cu²⁺) modification, and the convenience and wide applicability of this technique make it a promising strategy to avoid bacterial infections on implants.