Fucoidan sulfate from <i>Sargassum fusiforme</i> regulates the SARS-CoV-2 receptor AXL expression in human embryonic lung diploid fibroblast cells.

Xuqiang Zhou; Weihua Jin; Di Jiang; Yipeng XU; Sanying Wang; Xinna WU; Yunchuang Chang; Huili SU; Tianjun Zhu; Xiaogang XU; Genxiang Mao

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Fucoidan sulfate from Sargassum fusiforme regulates the SARS-CoV-2 receptor AXL expression in human embryonic lung diploid fibroblast cells.

Author: Xuqiang ZHOU ¹ ; Weihua JIN ² ; Di JIANG ² ; Yipeng XU ³ ; Sanying WANG ⁴ ; Xinna WU ⁵ ; Yunchuang CHANG ⁶ ; Huili SU ⁴ ; Tianjun ZHU ⁴ ; Xiaogang XU ⁷ ; Genxiang MAO ⁸
Author Information

1. College of Life Science, Zhejiang Chinese Medical University, Hangzhou 310053, China.
2. College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310014, China.
3. Zhejiang Cancer Hospital, the Key Laboratory of Zhejiang Province for Aptamers and Theranostics, Institute of Basic Medicine and Cancer (IBMC), Chinese Academy of Sciences, Hangzhou 310022, China.
4. Zhejiang Provincial Key Lab of Geriatrics & Geriatrics Institute of Zhejiang Province, Department of Geriatrics, Zhejiang Hospital, Hangzhou 310030, China.
5. School of Medicine, Zhejiang University, Hangzhou 310058, China.
6. School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, China.
7. Zhejiang Provincial Key Lab of Geriatrics & Geriatrics Institute of Zhejiang Province, Department of Geriatrics, Zhejiang Hospital, Hangzhou 310030, China. xuxg@zju.edu.cn.
8. College of Life Science, Zhejiang Chinese Medical University, Hangzhou 310053, China. xuxg@zju.edu.cn, maogenxiang@163.com.
Publication Type:Journal Article
MeSH: Humans; SARS-CoV-2; Sargassum/metabolism*; Diploidy; Sulfates/metabolism*; COVID-19; Polysaccharides/pharmacology*; Lung
From: Journal of Zhejiang University. Science. B 2023;24(11):1047-1052
CountryChina
Language:English
Abstract: 新冠病毒感染疫情严重威胁着世界各国人民的生命健康。目前，对病毒感染的防治研究主要集中在抑制病毒与分子受体的结合上。AXL作为新发现的严重急性呼吸综合征冠状病毒2型（SARS-CoV-2）受体，在协助病毒感染人体呼吸系统中发挥着重要作用，是未来临床干预的潜在靶点。本研究对已发表的单细胞测序数据进行整理和分析，发现AXL在年轻人肺细胞中的表达水平明显高于老年人。人胚肺二倍体成纤维细胞（2BS）是衰老研究的公认细胞株。本文采用2BS细胞构建复制性细胞衰老模型，发现年轻细胞中AXL的蛋白水平明显高于衰老细胞，据此推测年轻人感染的风险可能更高，需要注意防护。我们发现一种羊栖菜褐藻多糖硫酸酯组分（SFW-3）可显著下调年轻2BS细胞中AXL的表达水平，表明SFW-3具有一定的抗SARS-CoV-2感染的研究价值，同时表明2BS细胞株也可作为潜在的SARS-CoV-2体外感染模型。