Spinal P2X7R contributes to streptozotocin-induced mechanical allodynia in mice.
- Author:
Cheng-Ming NI
1
;
He-Ping SUN
2
;
Xiang XU
1
;
Bing-Yu LING
3
;
Hui JIN
1
;
Yu-Qiu ZHANG
4
;
Zhi-Qi ZHAO
4
;
Hong CAO
4
;
Lan XU
1
Author Information
- Publication Type:Journal Article
- Keywords: P2X7 receptor (P2X7R); Mechanical allodynia; Streptozotocin; Diabetic mice
- MeSH: Acetamides/pharmacology*; Animals; Diabetes Mellitus, Experimental/complications*; Diabetes Mellitus, Type 1/complications*; Diabetic Neuropathies/etiology*; Hyperalgesia/etiology*; Male; Mice; Mice, Inbred C57BL; Quinolines/pharmacology*; Receptors, Purinergic P2X7/physiology*; Spinal Cord/physiology*; Streptozocin/pharmacology*
- From: Journal of Zhejiang University. Science. B 2020;21(2):155-165
- CountryChina
- Language:English
- Abstract: Painful diabetic neuropathy (PDN) is a diabetes mellitus complication. Unfortunately, the mechanisms underlying PDN are still poorly understood. Adenosine triphosphate (ATP)-gated P2X7 receptor (P2X7R) plays a pivotal role in non-diabetic neuropathic pain, but little is known about its effects on streptozotocin (STZ)-induced peripheral neuropathy. Here, we explored whether spinal cord P2X7R was correlated with the generation of mechanical allodynia (MA) in STZ-induced type 1 diabetic neuropathy in mice. MA was assessed by measuring paw withdrawal thresholds and western blotting. Immunohistochemistry was applied to analyze the protein expression levels and localization of P2X7R. STZ-induced mice expressed increased P2X7R in the dorsal horn of the lumbar spinal cord during MA. Mice injected intrathecally with a selective antagonist of P2X7R and P2X7R knockout (KO) mice both presented attenuated progression of MA. Double-immunofluorescent labeling demonstrated that P2X7R-positive cells were mostly co-expressed with Iba1 (a microglia marker). Our results suggest that P2X7R plays an important role in the development of MA and could be used as a cellular target for treating PDN.
