Anti-senescence effect and molecular mechanism of the major royal jelly proteins on human embryonic lung fibroblast (HFL-I) cell line.

Chen-min Jiang; Xin Liu; Chun-xue LI; Hao-cheng Qian; Di Chen; Chao-qiang Lai; Li-rong Shen

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Anti-senescence effect and molecular mechanism of the major royal jelly proteins on human embryonic lung fibroblast (HFL-I) cell line.

Author: Chen-Min JIANG ¹ ; Xin LIU ¹ ; Chun-Xue LI ¹ ; Hao-Cheng QIAN ¹ ; Di CHEN ¹ ; Chao-Qiang LAI ² ; Li-Rong SHEN ¹
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1. College of Biosystems Engineering and Food Science, Zhejiang University / Key Laboratory of Agro-Products Postharvest Handling of Ministry of Agriculture and Rural Affairs / Zhejiang Key Laboratory for Agri-Food Processing, Hangzhou 310058, China.
2. USDA ARS Nutritional Genomics Laboratory, JM-USDA Human Nutrition Research Center on Aging at Tufts University, Boston, Massachusetts 02111, the United States.
Publication Type:Journal Article
Keywords: Major royal jelly protein; Human embryonic lung fibroblast (HFL-I) cell line; Anti-senescence; Relative proliferation activity; Telomere length; Molecular mechanism
MeSH: Animals; Bees; Cattle; Cell Line; Cell Proliferation; Cellular Senescence/drug effects*; Culture Media; Dose-Response Relationship, Drug; Fatty Acids/chemistry*; Fibroblasts/drug effects*; Humans; Insect Proteins/chemistry*; Lung/drug effects*; Serum Albumin/metabolism*; Spectrum Analysis, Raman; Superoxide Dismutase-1/metabolism*; TOR Serine-Threonine Kinases/metabolism*; Tumor Suppressor Protein p53/metabolism*; beta Catenin/metabolism*
From: Journal of Zhejiang University. Science. B 2018;19(12):960-972
CountryChina
Language:English
Abstract: Royal jelly (RJ) from honeybee has been widely used as a health promotion supplement. The major royal jelly proteins (MRJPs) have been identified as the functional component of RJ. However, the question of whether MRJPs have anti-senescence activity for human cells remains. Human embryonic lung fibroblast (HFL-I) cells were cultured in media containing no MRJPs (A), MRJPs at 0.1 mg/ml (B), 0.2 mg/ml (C), or 0.3 mg/ml (D), or bovine serum albumin (BSA) at 0.2 mg/ml (E). The mean population doubling levels of cells in media B, C, D, and E were increased by 12.4%, 31.2%, 24.0%, and 10.4%, respectively, compared with that in medium A. The cells in medium C also exhibited the highest relative proliferation activity, the lowest senescence, and the longest telomeres. Moreover, MRJPs up-regulated the expression of superoxide dismutase-1 (SOD1) and down-regulated the expression of mammalian target of rapamycin (MTOR), catenin beta like-1 (CTNNB1), and tumor protein p53 (TP53). Raman spectra analysis showed that there were two unique bands related to DNA synthesis materials, amide carbonyl group vibrations and aromatic hydrogens. These results suggest that MRJPs possess anti-senescence activity for the HFL-I cell line, and provide new knowledge illustrating the molecular mechanism of MRJPs as anti-senescence factors.