Dual flow immunochromatographic assay for rapid and simultaneous quantitative detection of ochratoxin A and zearalenone in corn, wheat, and feed samples.
- Author:
Xian ZHANG
1
;
Ke HE
1
;
Yun FANG
2
;
Tong CAO
3
;
Narayan PAUDYAL
3
;
Xiao-Feng ZHANG
2
;
Hou-Hui SONG
1
;
Xiao-Liang LI
3
;
Wei-Huan FANG
1
Author Information
- Publication Type:Journal Article
- Keywords: Immunochromatographic assay; Gold nanoparticles; Ochratoxin A; Zearalenone; Quantification
- MeSH: Animal Feed; Calibration; Chromatography, Affinity; Chromatography, Liquid; Colloids; Food Contamination/analysis*; Food Safety; Gold; Immunoassay/methods*; Inhibitory Concentration 50; Limit of Detection; Metal Nanoparticles; Ochratoxins/analysis*; Regression Analysis; Reproducibility of Results; Sensitivity and Specificity; Triticum; Zea mays; Zearalenone/analysis*
- From: Journal of Zhejiang University. Science. B 2018;19(11):871-883
- CountryChina
- Language:English
- Abstract: A one-step dual flow immunochromatographic assay (DICGA), based on a competitive format, was developed for simultaneous quantification of ochratoxin A (OTA) and zearalenone (ZEN) in corn, wheat, and feed samples. The limit of detection for OTA was 0.32 ng/ml with a detection range of 0.53‒12.16 ng/ml, while for ZEN it was 0.58 ng/ml with a detection range of 1.06‒39.72 ng/ml. The recovery rates in corn, wheat, and feed samples ranged from 77.3% to 106.3% with the coefficient of variation lower than 15%. Naturally contaminated corn, wheat, and feed samples were analyzed using both DICGA and liquid chromatography-tandem mass spectrometry (LC-MS/MS) and the correlation between the two methods was evaluated using a regression analysis. The DICGA method shows great potential for simple, rapid, sensitive, and cost-effective quantitative detection of OTA and ZEN in food safety control.
