Glycyrrhizic acid activates chicken macrophages and enhances their Salmonella-killing capacity in vitro.

Bai-kui Wang; Yu-long Mao; Li Gong; Xin XU; Shou-qun Jiang; Yi-bing Wang; Wei-fen LI

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Glycyrrhizic acid activates chicken macrophages and enhances their Salmonella-killing capacity in vitro.

Author: Bai-Kui WANG ¹ ; Yu-Long MAO ¹ ; Li GONG ¹ ; Xin XU ¹ ; Shou-Qun JIANG ² ; Yi-Bing WANG ¹ ; Wei-Fen LI ¹
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1. Key Laboratory of Animal Molecular Nutrition of Education of Ministry, Key Laboratory of Animal Feed and Nutrition of Zhejiang Province, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.
2. Institute of Animal Science, Guangdong Academy of Agricultural Sciences, State Key Laboratory of Livestock and Poultry Breeding, Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture, Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangdong Key Laboratory of Animal Breeding and Nutrition, Guangzhou 510640, China.
Publication Type:Journal Article
Keywords: Glycyrrhizic acid; Chicken macrophage; Macrophage activation; Salmonella Typhimurium; Nuclear factor κB (NF-κB); c-Jun N-terminal kinase (JNK)
MeSH: Animals; Cells, Cultured; Chickens; Glycyrrhizic Acid/pharmacology*; Macrophage Activation/drug effects*; NF-kappa B/physiology*; Phagocytosis/drug effects*; Salmonella/drug effects*; Signal Transduction/drug effects*
From: Journal of Zhejiang University. Science. B 2018;19(10):785-795
CountryChina
Language:English
Abstract: OBJECTIVE:Salmonella enterica remains a major cause of food-borne disease in humans, and Salmonella Typhimurium (ST) contamination of poultry products is a worldwide problem. Since macrophages play an essential role in controlling Salmonella infection, the aim of this study was to evaluate the effect of glycyrrhizic acid (GA) on immune function of chicken HD11 macrophages.
METHODS:Chicken HD11 macrophages were treated with GA (0, 12.5, 25, 50, 100, 200, 400, or 800 μg/ml) and lipopolysaccharide (LPS, 500 ng/ml) for 3, 6, 12, 24, or 48 h. Evaluated responses included phagocytosis, bacteria-killing, gene expression of cell surface molecules (cluster of differentiation 40 (CD40), CD80, CD83, and CD197) and antimicrobial effectors (inducible nitric oxide synthase (iNOS), NADPH oxidase-1 (NOX-1), interferon-γ (IFN-γ), LPS-induced tumor necrosis factor (TNF)-α factor (LITAF), interleukin-6 (IL-6), and IL-10), and production of nitric oxide (NO) and hydrogen peroxide (H₂O₂).
RESULTS:GA increased the internalization of both fluorescein isothiocyanate (FITC)-dextran and ST by HD11 cells and markedly decreased the intracellular survival of ST. We found that the messenger RNA (mRNA) expression of cell surface molecules (CD40, CD80, CD83, and CD197) and cytokines (IFN-γ, IL-6, and IL-10) of HD11 cells was up-regulated following GA exposure. The expression of iNOS and NOX-1 was induced by GA and thereby the productions of NO and H₂O₂ in HD11 cells were enhanced. Notably, it was verified that nuclear factor-κB (NF-κB) and c-Jun N-terminal kinase (JNK) pathways were responsible for GA-induced synthesis of NO and IFN-γ gene expression.
CONCLUSIONS:Taken together, these results suggested that GA exhibits a potent immune regulatory effect to activate chicken macrophages and enhances Salmonella-killing capacity.