Cellular stress and redox activity proteins are involved in gastric carcinogenesis associated with Helicobacter pylori infection expressing high levels of thioredoxin-1.
- Author:
Yan-Yan SHI
1
;
Jing ZHANG
2
;
Ting ZHANG
3
;
Man ZHOU
4
;
Ye WANG
2
;
He-Jun ZHANG
2
;
Shi-Gang DING
2
Author Information
- Publication Type:Journal Article
- Keywords: Thioredoxin-1 (Trx1); Helicobacter pylori; Gastric carcinogenesis; Proteomics; Isobaric tags for relative and absolute quantitation (iTRAQ)
- MeSH: 14-3-3 Proteins/physiology*; Animals; Computational Biology; Gerbillinae; Glutathione Transferase/physiology*; HSP70 Heat-Shock Proteins/physiology*; Helicobacter Infections/complications*; Helicobacter pylori; Oxidation-Reduction; Stomach Neoplasms/etiology*; Stress, Physiological; Thioredoxins/physiology*
- From: Journal of Zhejiang University. Science. B 2018;19(10):750-763
- CountryChina
- Language:English
- Abstract: Helicobacter pylori infection is related to the development of gastric diseases. Our previous studies showed that high thioredoxin-1 (Trx1) expression in H. pylori can promote gastric carcinogenesis. To explore the underlying molecular mechanisms, we performed an isobaric tags for relative and absolute quantitation (iTRAQ)-based quantitative proteomic analysis of stomach tissues from Mongolian gerbil infected with H. pylori expressing high and low Trx1. Differences in the profiles of the expressed proteins were analyzed by bioinformatics and verified using Western blot analysis. We found three candidate proteins, 14-3-3α/β, glutathione-S-transferase (GST), and heat shock protein 70 (HSP70), in high Trx1 tissues compared with low Trx1 tissues and concluded that cellular stress and redox activity-related proteins were involved in the pathogenesis of gastric cancer associated with H. pylori Trx1.