Ureaplasma urealyticum-derived lipid-associated membrane proteins introduce IL-6, IL-8, and TNF-α cytokines into human amniotic epithelial cells via Toll-like receptor 2.
- Author:
Guang-Yong YE
1
;
Ke-Yi WANG
2
;
Qiao-di GUI
3
;
Min WANG
1
Author Information
- Publication Type:Journal Article
- Keywords: Ureaplasma urealyticum; Lipid-associated membrane protein; Human amniotic epithelial cell; Toll-like receptor 2
- MeSH: Amnion/cytology*; Amniotic Fluid/cytology*; Cytokines/metabolism*; Dose-Response Relationship, Drug; Epithelial Cells/metabolism*; Female; Humans; Inflammation; Interleukin-6/metabolism*; Interleukin-8/metabolism*; Lipids/chemistry*; Lipopolysaccharides/metabolism*; Membrane Proteins/metabolism*; Placenta/metabolism*; Pregnancy; Toll-Like Receptor 2/metabolism*; Tumor Necrosis Factor-alpha/metabolism*; Up-Regulation; Ureaplasma urealyticum/metabolism*
- From: Journal of Zhejiang University. Science. B 2018;19(8):654-661
- CountryChina
- Language:English
-
Abstract:
OBJECTIVE:The purpose of this study was to determine the role of Ureaplasma urealyticum-derived lipid-associated membrane proteins (LAMPs) in the host innate immune system, specifically their effect on Toll-like receptors (TLRs).
METHODS:LAMPs were derived from U. urealyticum strains, and human amniotic epithelial cells (HAECs) were isolated from healthy full-term placentas. Cytokine concentrations were determined by enzyme-linked immunosorbent assay (ELISA) and TLR2 mRNA by real-time PCR. Expression of TLR2 was confirmed by Western blotting and immunohistochemistry.
RESULTS:LAMPs induced HAECs to produce inflammatory cytokines interleukin (IL)-6, IL-8, and tumor necrosis factor (TNF)-α. Cytokine production was reduced after blocking TLR2 using TLR2 inhibitor (anti-hTLR2-IgA).
CONCLUSIONS:LAMPs isolated from U. urealyticum induced TLR2-dependent up-regulation of inflammatory genes and cytokines in HAECs.
