Biosynthesis of (R)-2-hydroxy-3-phenylpropionic acid using whole recombinant Escherichia coli cells in an aqueous/n-octane biphasic system.
- Author:
Yi-Bo ZHU
1
;
Yan XU
1
;
Li-Mei WANG
1
;
Bin QI
1
Author Information
- Publication Type:Journal Article
- Keywords: (R)-2-hydroxy-3-phenylpropionic acid; Phenylpyruvate; Aqueous/n-octane biphasic system; Whole cell bioconversion; Recombinant Escherichia coli
- MeSH: Buffers; Escherichia coli/metabolism*; L-Lactate Dehydrogenase/metabolism*; Microorganisms, Genetically-Modified; Octanes/chemistry*; Phenylpropionates/chemistry*; Recombinant Proteins/chemistry*; Solvents/chemistry*; Stress, Mechanical; Temperature
- From: Journal of Zhejiang University. Science. B 2018;19(4):285-292
- CountryChina
- Language:English
- Abstract: (R)-2-hydroxy-3-phenylpropionic acid (PLA) is an ideal antimicrobial compound with broad-spectrum activity against a wide range of Gram-positive bacteria, some Gram-negative bacteria, and fungi. We studied the bioconversion of phenylpyruvate (PPA) to PLA using whole recombinant Escherichia coli cells in a series of buffer/organic solvent systems. Octane was found to be the best organic solvent. The optimum volume ratio of the water phase to the n-octane phase, conversion temperature, substrate concentration, and cell concentration were 6:4, 40 °C, 12.5 g/L, and 30 g/L wet cells, respectively. Under the optimized conditions, the average PLA productivity in the aqueous/ n-octane system was 30.69% higher than that in the aqueous system, and 32.31 g/L PLA was obtained with the use of a stirred reactor (2-L scale). Taken together, our findings indicated that PLA biosynthesis was more efficient in an aqueous/n-octane biphasic system than in a monophasic aqueous system. The proposed biphasic system is an effective strategy for enhancing PLA yield and the biosynthesis of its analogues.