In vitro antioxidant activity of phenolic-enriched extracts from Zhangping Narcissus tea cake and their inhibition on growth and metastatic capacity of 4T1 murine breast cancer cells.

Le Ying; De-dong Kong; Yuan-yuan Gao; Feng Yan; Yue-fei Wang; Ping XU

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In vitro antioxidant activity of phenolic-enriched extracts from Zhangping Narcissus tea cake and their inhibition on growth and metastatic capacity of 4T1 murine breast cancer cells.

Author: Le YING ¹ ; De-Dong KONG ¹ ; Yuan-Yuan GAO ¹ ; Feng YAN ² ; Yue-Fei WANG ¹ ; Ping XU ¹
Author Information

1. Department of Tea Science, Zhejiang University, Hangzhou 310058, China.
2. Department of Molecular and Translational Science, Monash University, Clayton, Victoria 3168, Australia.
Publication Type:Journal Article
Keywords: Oolong tea; Antioxidant activity; Metastasis; Cell cycle
MeSH: Animals; Antineoplastic Agents, Phytogenic/pharmacology*; Antioxidants/pharmacology*; Camellia sinensis/chemistry*; Cell Line, Tumor; Cell Movement/drug effects*; Cell Proliferation/drug effects*; Female; Mammary Neoplasms, Experimental/pathology*; Mice; Neoplasm Metastasis; Phenols/pharmacology*; Plant Extracts/pharmacology*
From: Journal of Zhejiang University. Science. B 2018;19(3):199-210
CountryChina
Language:English
Abstract: Phenolics, as the main bioactive compounds in tea, have been suggested to have potential in the prevention of various human diseases. However, little is known about phenolics and their bioactivity in Zhangping Narcissue tea cake which is considered the most special kind of oolong tea. To unveil its bioactivity, three phenolic-enriched extracts were obtained from Zhangping Narcissue tea cake using ethyl acetate, n-butanol, and water. Their main chemical compositions and in vitro bioactivity were analyzed by high-performance liquid chromatography (HPLC) and ultra-performance liquid chromatography-mass spectrometry (UPLC-MS). The ethyl acetate fraction (ZEF) consisted of higher content of phenolics, flavonoids, procyanidins, and catechin monomers (including epigallocatechin gallate (EGCG), epicatechin gallate (ECG), and gallocatechin gallate (GCG)) than n-butanol fraction (ZBF) and water fraction (ZWF). ZEF exhibited the strongest antioxidant capacity in vitro due to its abundant bioactive compounds. This was validated by Pearson correlation and hierarchical clustering analyses. ZEF also showed a remarkable inhibition on the growth, migration, and invasion of 4T1 murine breast cancer cells.