Toxicity testing of four silver nanoparticle-coated dental castings in 3-D LO2 cell cultures.
- Author:
Yi-Ying ZHAO
1
;
Qiang CHU
1
;
Xu-Er SHI
1
;
Xiao-Dong ZHENG
1
;
Xiao-Ting SHEN
2
;
Yan-Zhen ZHANG
3
Author Information
1. Department of Food Science and Nutrition, Zhejiang Key Laboratory for Agro-Food Processing, Fuli Institute of Food Science, Hangzhou 310058, China.
2. Huajiachi Dental Center, Stomatology Hospital Affiliated to Zhejiang University of Medicine, Hangzhou 310006, China.
3. Department of General Dentistry, the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310012, China.
- Publication Type:Journal Article
- Keywords:
LO2 cell;
3-D model;
Silver nanoparticles;
Dental alloys;
Toxicity test
- MeSH:
Cells, Cultured;
Dental Casting Technique;
Hepatocytes/drug effects*;
Humans;
Metal Nanoparticles/toxicity*;
Silver/toxicity*;
Toxicity Tests
- From:
Journal of Zhejiang University. Science. B
2018;19(2):159-167
- CountryChina
- Language:English
-
Abstract:
To address the controversial issue of the toxicity of dental alloys and silver nanoparticles in medical applications, an in vivo-like LO2 3-D model was constructed within polyvinylidene fluoride hollow fiber materials to mimic the microenvironment of liver tissue. The use of microscopy methods and the measurement of liver-specific functions optimized the model for best cell performances and also proved the superiority of the 3-D LO2 model when compared with the traditional monolayer model. Toxicity tests were conducted using the newly constructed model, finding that four dental castings coated with silver nanoparticles were toxic to human hepatocytes after cell viability assays. In general, the toxicity of both the castings and the coated silver nanoparticles aggravated as time increased, yet the nanoparticles attenuated the general toxicity by preventing metal ion release, especially at high concentrations.
