Physical interactions and mutational analysis of MoYpt7 in Magnaporthe oryzae.

Author: Lu-Yao HUANG ¹ ; Min WU ¹ ; Xiao-Yun YU ² ; Lin LI ¹ ; Fu-Cheng LIN ¹ ; Xiao-Hong LIU ¹
Author Information

1. State Key Laboratory for Rice Biology, Biotechnology Institute, Zhejiang University, Hangzhou 310058, China.
2. Huangdao Entry-Exit Inspection and Quarantine Bureau, Huangdao 266555, China.
Publication Type:Journal Article
Keywords: Bimolecular fluorescence complementation; Immunoprecipitation; MoGdi1; MoYpt7
MeSH: DNA Mutational Analysis; Fungal Proteins/metabolism*; Gene Expression Regulation, Fungal; Genes, Fungal; Green Fluorescent Proteins/metabolism*; Magnaporthe/genetics*; Microscopy, Fluorescence; Mutation; Oryza/microbiology*; Phenotype; Plant Diseases/microbiology*; Protein Isoforms
From: Journal of Zhejiang University. Science. B 2018;19(1):79-84
CountryChina
Language:English
Abstract: In this study, we analyzed the physical interactions of the dominant negative isoform of MoYpt7. Our results show that MoYpt7 interacts with MoGdi1. The dominant negative isoform of MoYpt7 (dominant negative isoform, N125I) is essential for colony morphology, conidiation, and pathogenicity in the rice blast fungus. These results further demonstrate the biological functions of MoYpt7 in Magnaporthe oryzae.