Polysaccharide of Alocasia cucullata Exerts Antitumor Effect by Regulating Bcl-2, Caspase-3 and ERK1/2 Expressions during Long-Time Administration.
10.1007/s11655-023-3700-6
- Author:
Qi-Chun ZHOU
1
;
Shi-Lin XIAO
1
;
Ru-Kun LIN
1
;
Chan LI
1
;
Zhi-Jie CHEN
1
;
Yi-Fei CHEN
1
;
Chao-Hua LUO
1
;
Zhi-Xian MO
2
;
Ying-Bo LIN
3
Author Information
1. School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, 510515, China.
2. School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, 510515, China. cherrymo@smu.edu.cn.
3. School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, 510515, China. 1455326563@qq.com.
- Publication Type:Journal Article
- Keywords:
Bcl-2;
Caspase-3;
Chinese medicine;
ERK1/2;
polysaccharide of Alocasia cucullata;
tumor cells
- MeSH:
Mice;
Animals;
Alocasia/metabolism*;
MAP Kinase Signaling System;
Caspase 3/metabolism*;
Apoptosis;
RNA, Messenger/metabolism*
- From:
Chinese journal of integrative medicine
2024;30(1):52-61
- CountryChina
- Language:English
-
Abstract:
OBJECTIVE:To study the in vitro and in vivo antitumor effects of the polysaccharide of Alocasia cucullata (PAC) and the underlying mechanism.
METHODS:B16F10 and 4T1 cells were cultured with PAC of 40 µg/mL, and PAC was withdrawn after 40 days of administration. The cell viability was detected by cell counting kit-8. The expression of Bcl-2 and Caspase-3 proteins were detected by Western blot and the expressions of ERK1/2 mRNA were detected by quantitative real-time polymerase chain reaction (qRT-PCR). A mouse melanoma model was established to study the effect of PAC during long-time administration. Mice were divided into 3 treatment groups: control group treated with saline water, positive control group (LNT group) treated with lentinan at 100 mg/(kg·d), and PAC group treated with PAC at 120 mg/(kg·d). The pathological changes of tumor tissues were observed by hematoxylin-eosin staining. The apoptosis of tumor tissues was detected by TUNEL staining. Bcl-2 and Caspase-3 protein expressions were detected by immunohistochemistry, and the expressions of ERK1/2, JNK1 and p38 mRNA were detected by qRT-PCR.
RESULTS:In vitro, no strong inhibitory effects of PAC were found in various tumor cells after 48 or 72 h of administration. Interestingly however, after 40 days of cultivation under PAC, an inhibitory effect on B16F10 cells was found. Correspondingly, the long-time administration of PAC led to downregulation of Bcl-2 protein (P<0.05), up-regulation of Caspase-3 protein (P<0.05) and ERK1 mRNA (P<0.05) in B16F10 cells. The above results were verified by in vivo experiments. In addition, viability of B16F10 cells under long-time administration culture in vitro decreased after drug withdrawal, and similar results were also observed in 4T1 cells.
CONCLUSIONS:Long-time administration of PAC can significantly inhibit viability and promote apoptosis of tumor cells, and had obvious antitumor effect in tumor-bearing mice.
