Asperuloside Promotes Apoptosis of Cervical Cancer Cells through Endoplasmic Reticulum Stress-Mitochondrial Pathway.
10.1007/s11655-023-3695-z
- Author:
Zhi-Min QI
1
;
Xia WANG
2
;
Xia LIU
2
;
Juan ZHAO
3
Author Information
1. Medical Examination Center, Hebei Province Hospital of Chinese Medicine, Shijiazhuang, 050011, China.
2. Department of Gynaecology and Obstetrics, Hebei Province Hospital of Chinese Medicine, Shijiazhuang, 050011, China.
3. Experimental Center for Teaching, Hebei Medical University, Shijiazhuang, 050017, China. 16800658@hebmu.edu.cn.
- Publication Type:Journal Article
- Keywords:
Chinese medicine;
apoptosis;
asperuloside;
cervical cancer;
endoplasmic reticulum stress;
mitochondrial damage
- MeSH:
Female;
Humans;
Uterine Cervical Neoplasms/metabolism*;
Caspase 3/metabolism*;
bcl-2-Associated X Protein/metabolism*;
Reactive Oxygen Species/metabolism*;
Endoplasmic Reticulum Chaperone BiP;
HeLa Cells;
Proto-Oncogene Proteins c-bcl-2/metabolism*;
Apoptosis;
Endoplasmic Reticulum Stress;
Cell Line, Tumor
- From:
Chinese journal of integrative medicine
2024;30(1):34-41
- CountryChina
- Language:English
-
Abstract:
OBJECTIVE:To investigate the effects of asperuloside on cervical cancer based on endoplasmic reticulum (ER) stress and mitochondrial pathway.
METHODS:Different doses (12.5-800 µg/mL) of asperuloside were used to treat cervical cancer cell lines Hela and CaSki to calculate the half maximal inhibitory concentration (IC50) of asperuloside. The cell proliferation was analyzed by clone formation assay. Cell apoptosis, intracellular reactive oxygen species (ROS) and mitochondrial membrane potential were determined by flow cytometry. The protein expressions of cleaved-caspase-3, Bcl-2, Bax, Cyt-c, cleaved-caspase-4 and glucose-regulated protein 78 (GRP78) were analyzed by Western blot. And the inhibitor of ER stress, 4-phenyl butyric acid (4-PBA) was used to treat cervical cancer cells to further verify the role of ER stress in the apoptosis of cervical cancer cells induced by asperuloside.
RESULTS:Asperuloside of 325, 650, and 1300 µg/mL significantly inhibited the proliferation and promoted apoptosis of Hela and CaSki cells (P<0.01). All doses of asperuloside significantly increased intracellular ROS levels, reduced mitochondrial membrane potential, significantly reduced Bcl-2 protein expression level, and increased Bax, Cyt-c, GRP78 and cleaved-caspase-4 expressions (P<0.01). In addition, 10 mmol/L 4-PBA treatment significantly promoted cell proliferation and reduced apoptosis (P<0.05), and 650 µg/mL asperuloside could reverse 4-PBA-induced increased cell proliferation, decreased apoptosis and cleaved-caspase-3, -4 and GRP78 protein expressions (P<0.05).
CONCLUSION:Our study revealed the role of asperuloside in cervical cancer, suggesting that asperuloside promotes apoptosis of cervical cancer cells through ER stress-mitochondrial pathway.
