Radix Panacis quinquefolii Extract Ameliorates Inflammatory Bowel Disease through Inhibiting Inflammation.
10.1007/s11655-022-3543-6
- Author:
Xi-Xin WANG
1
;
Hong-Yuan ZOU
1
;
Yong-Na CAO
1
;
Xuan-Ming ZHANG
1
;
Meng SUN
1
;
Peng-Fei TU
2
;
Ke-Chun LIU
1
;
Yun ZHANG
3
Author Information
1. Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250103, China.
2. State Key Laboratory of Natural Medicines and Biomimetic Medicines, School of Pharmacy, Peking University, Beijing, 100191, China.
3. Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250103, China. xiaohan_0818@163.com.
- Publication Type:Journal Article
- Keywords:
Radix Panacis quinguefolii root extract;
anti-inflammatory;
inflammatory bowel disease;
zebrafish
- MeSH:
Animals;
Zebrafish;
Lipopolysaccharides;
Disease Models, Animal;
Inflammatory Bowel Diseases/metabolism*;
Inflammation/drug therapy*;
Anti-Inflammatory Agents/therapeutic use*;
Trinitrobenzenesulfonic Acid/adverse effects*;
Colitis/drug therapy*
- From:
Chinese journal of integrative medicine
2023;29(9):825-831
- CountryChina
- Language:English
-
Abstract:
OBJECTIVE:To investigate the anti-inflammatory activity of Radix Panacis quinguefolii root extract (RPQE) and its therapeutic effects on inflammatory bowel disease (IBD).
METHODS:The 72-hour post-fertilization zebrafish was used to generate the local and systematic inflammation models through tail-amputation and lipopolysaccharide (LPS)-induction (100 µ g/mL), respectively. The Tg(zlyz:EGFP) zebrafish was induced with 75 µ g/mL 2,4,6-trinitrobenzene sulfonic acid (TNBS) for establishing the IBD model. The tail-amputated, LPS-, and TNBS-induced models were subjected to RPQE (ethanol fraction, 10-20 µ g/mL) administration for 12 and 24 h, respectively. Anti-inflammatory activity of RPQE was evaluated by detecting migration and aggregation of leukocytes and expression of inflammation-related genes. Meanwhile, TNBS-induced fish were immersed in 0.2% (W/V) calcein for 1.5 h and RPQE for 12 h before photographing to analyze the intestinal efflux efficiency (IEE). Moreover, the expression of inflammation-related genes in these fish was detected by quantitative polymerase chain reaction.
RESULTS:Subject to RPQE administration, the migration and aggregation of leukocytes were significantly alleviated in 3 zebrafish models (P<0.01). Herein, RPQE ameliorated TNBS-induced IBD with respect to a significantly reduced number of leukocytes, improved IEE, and inhibited gene expression of pro-inflammatory factors (P<0.05 or P<0.01).
CONCLUSION:RPQE exhibited therapeutic effects on IBD by inhibiting inflammation.
