Identification and Analysis of SND1 as an Oncogene and Prognostic Biomarker for Lung Adenocarcinoma
10.3779/j.issn.1009-3419.2023.102.47
- VernacularTitle:SND1作为肺腺癌癌基因和预后标志物的鉴定与分析
- Author:
ZHANG RUIHAO
1
;
HUANG HUA
;
ZHU GUANGSHENG
;
WU DI
;
CHEN CHEN
;
CAO PEIJUN
;
DING CHEN
;
LIU HONGYU
;
CHEN JUN
;
LI YONGWEN
Author Information
1. 300052 天津,天津医科大学总医院肺部肿瘤外科
- Keywords:
SND1;
Lung neoplasms;
Cell cycle;
Immune infiltration
- From:
Chinese Journal of Lung Cancer
2024;27(1):25-37
- CountryChina
- Language:Chinese
-
Abstract:
Background and objective Transcription factor(TF)can bind specific sequences that either promotes or represses the transcription of target genes,and exerts important effects on tumorigenesis,migration,invasion.Staphylococcal nuclease-containing structural domain 1(SND1),which is a transcriptional co-activator,is considered as a promising target for tumor therapy.However,its role in lung adenocarcinoma(LUAD)remains unclear.This study aims to explore the role of SND1 in LUAD.Methods Data from The Cancer Genome Atlas(TCGA),Gene Expression Omnibus(GEO),Clinical Pro-teomic Tumor Analysis Consortium(CPTAC),and Human Protein Atlas(HPA)database was obtained to explore the associa-tion between SND1 and the prognosis,as well as the immune cell infiltration,and subcellular localization in LUAD tissues.Furthermore,the functional role of SND1 in LUAD was verified in vitro.EdU assay,CCK-8 assay,flow cytometry,scratch assay,Transwell assay and Western blot were performed.Results SND1 was found to be upregulated and high expression of SND1 is correlated with poor prognosis of LUAD patients.In addition,SND1 was predominantly present in the cytoplasm of LUAD cells.Enrichment analysis showed that SND1 was closely associated with the cell cycle,as well as DNA replication,and chro-mosome segregation.Immune infiltration analysis showed that SND1 was closely associated with various immune cell popula-tions,including T cells,B cells,cytotoxic cells and dendritic cells.In vitro studies demonstrated that silencing of SND1 inhib-ited cell proliferation,invasion and migration of LUAD cells.Besides,cell cycle was blocked at G,phase by down-regulating SND1.Conclusion SND1 might be an important prognostic biomarker of LUAD and may promote LUAD cells proliferation and migration.
