Identification and Analysis of SND1 as an Oncogene and Prognostic Biomarker
for Lung Adenocarcinoma.
10.3779/j.issn.1009-3419.2023.102.47
- Author:
Ruihao ZHANG
1
;
Hua HUANG
1
;
Guangsheng ZHU
1
;
Di WU
1
;
Chen CHEN
2
;
Peijun CAO
1
;
Chen DING
3
;
Hongyu LIU
2
;
Jun CHEN
1
;
Yongwen LI
2
Author Information
1. Department of Lung Cancer Surgery, Tianjin Medical University General Hospital, Tianjin 300052, China.
2. Tianjin Key Laboratory of Lung Cancer
Metastasis and Tumor Microenvironment, Tianjin Lung Cancer Institute, Tianjin Medical University General Hospital,
Tianjin 300052, China.
3. Department of Thoracic Surgery, First Affiliated Hospital, School of Medicine, Shihezi University,
Shihezi 832003, China.
- Publication Type:Journal Article
- Keywords:
Cell cycle;
Immune infiltration;
Lung neoplasms;
SND1
- MeSH:
Humans;
Prognosis;
Proteomics;
Lung Neoplasms/genetics*;
Oncogenes;
Adenocarcinoma of Lung/genetics*;
Biomarkers;
Endonucleases/genetics*
- From:
Chinese Journal of Lung Cancer
2024;27(1):25-37
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Transcription factor (TF) can bind specific sequences that either promotes or represses the transcription of target genes, and exerts important effects on tumorigenesis, migration, invasion. Staphylococcal nuclease-containing structural domain 1 (SND1), which is a transcriptional co-activator, is considered as a promising target for tumor therapy. However, its role in lung adenocarcinoma (LUAD) remains unclear. This study aims to explore the role of SND1 in LUAD.
METHODS:Data from The Cancer Genome Atlas (TCGA), Gene Expression Omnibus (GEO), Clinical Proteomic Tumor Analysis Consortium (CPTAC), and Human Protein Atlas (HPA) database was obtained to explore the association between SND1 and the prognosis, as well as the immune cell infiltration, and subcellular localization in LUAD tissues. Furthermore, the functional role of SND1 in LUAD was verified in vitro. EdU assay, CCK-8 assay, flow cytometry, scratch assay, Transwell assay and Western blot were performed.
RESULTS:SND1 was found to be upregulated and high expression of SND1 is correlated with poor prognosis of LUAD patients. In addition, SND1 was predominantly present in the cytoplasm of LUAD cells. Enrichment analysis showed that SND1 was closely associated with the cell cycle, as well as DNA replication, and chromosome segregation. Immune infiltration analysis showed that SND1 was closely associated with various immune cell populations, including T cells, B cells, cytotoxic cells and dendritic cells. In vitro studies demonstrated that silencing of SND1 inhibited cell proliferation, invasion and migration of LUAD cells. Besides, cell cycle was blocked at G1 phase by down-regulating SND1.
CONCLUSIONS:SND1 might be an important prognostic biomarker of LUAD and may promote LUAD cells proliferation and migration.
