Application of Rapid HE Staining in Cytological Rapid On-site Evaluation of
Peripheral Lung Cancer Needle Biopsy.
10.3779/j.issn.1009-3419.2023.101.24
- Author:
Jian HE
1
;
Guilan XIA
2
;
Shiping WANG
3
;
Kun CHEN
4
Author Information
1. Department of Pulmonary and Critical Care Medicine, Anning First People's Hospital, Kunming 650300, China.
2. Department of Pathology, Anning First People's Hospital, Kunming 650300, China.
3. Imaging Medical Center, Anning First People's Hospital, Kunming 650300, China.
4. Department of Laboratory Medicine, Baoshan District of Huashan Hospital, Fudan University, Shanghai 200040, China.
- Publication Type:Journal Article
- Keywords:
CT-guided lung biopsy;
Cytological rapid on-site evaluation;
Lung neoplasms;
Peripheral lung cancer;
Rapid HE staining
- MeSH:
Humans;
Lung Neoplasms/pathology*;
Eosine Yellowish-(YS);
Rapid On-site Evaluation;
Biopsy, Needle/methods*;
Staining and Labeling
- From:
Chinese Journal of Lung Cancer
2023;26(8):572-578
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Rapid on-site evaluation (ROSE) is a technique used for simultaneous evaluation of biopsy specimens through rapid cytology staining. Diff-Quik (DQ) staining is the most commonly employed method for cytological rapid on-site evaluation (C-ROSE). However, the utilization of DQ staining for on-site cytological interpretation remains uncommon among pathologists in China, posing challenges to the implementation of C-ROSE. This study aims to assess the application of rapid hematoxylin-eosin (HE) staining and DQ staining for C-ROSE during percutaneous needle biopsy of peripheral lung cancer and evaluate the value of rapid HE staining in C-ROSE.
METHODS:Computed tomography (CT)-guided lung biopsies were conducted on 300 patients diagnosed with peripheral lung cancer. The patients were randomly assigned to two groups for C-ROSE using either rapid HE staining or DQ staining, and subsequently the two methods were compared and evaluated.
RESULTS:The concordance rate between C-ROSE and histopathological diagnosis was 96.7%. The median staining time for rapid HE staining was 160 s, while that for DQ staining was 120 s, representing a significant difference between the two groups (P<0.001). However, there were no significant differences observed in terms of total biopsy time, concordance rate with histopathology, cytology specimen peeling rate, and incidence of serious adverse reactions between the two groups (P>0.05).
CONCLUSIONS:Both staining methods comply with C-ROSE criteria in the biopsy setting of peripheral lung cancer. Rapid HE staining is more aligned with domestic clinical requirements and holds potential for further promotion and adoption in C-ROSE.
