Establishment of Patient-Derived Xenograft (PDX) Zebrafish Model of Multiple Myeloma and Its Application in Drug Screening.
10.19746/j.cnki.issn.1009-2137.2023.06.022
- Author:
Zhen YU
1
;
Ying LI
1
;
Ke-Fei WANG
1
;
Lu WANG
1
;
Mu HAO
2
Author Information
1. State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Haihe Laboratory of Cell Ecosystem, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China.
2. State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Haihe Laboratory of Cell Ecosystem, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China,E-mail: haomu@ihcams.ac.cn.
- Publication Type:Journal Article
- Keywords:
Bortezomib;
indirubin-3’-monoxime;
multiple myeloma;
patient-derived tumor xenograft model;
zebrafish
- MeSH:
Animals;
Humans;
Bortezomib/therapeutic use*;
Cell Line, Tumor;
Disease Models, Animal;
Drug Evaluation, Preclinical;
Heterografts;
Multiple Myeloma/pathology*;
Xenograft Model Antitumor Assays;
Zebrafish
- From:
Journal of Experimental Hematology
2023;31(6):1745-1749
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a MM patient-derived tumor xenograft model (MM-PDX) in zebrafish, and to evaluate the anti-myeloma activity of indirubin-3'-monoxime(I3MO) using this model.
METHODS:Zebrafish embryos 2 days after fertilization were transplanted with fluorescence labeled myeloma primary tumor cells, the survival of primary tumor cells in zebrafish was observed at 0,16 and 24 hours after cell injection. The zebrafish embryos after tumor cell transplantation were randomly divided into control group, BTZ treatment and I3MO treatment group. Before and 24 hours after treatment with BTZ and I3MO, the positive area with calcein or Dil in zebrafish were observed under fluorescence microscope to reflect the survival of tumor cells, and it was verified.
RESULTS:MM patient derived tumor cells survived in zebrafish. The construction of MM-PDX was successful. Compared with control group, the fluo- rescence area of the BTZ and I3MO treatment groups in zebrafish were significantly decreased(P<0.05), and BTZ and I3MO significantly inhibited the survival of MM cells in zebrafish.
CONCLUSION:MM-PDX model was successfully established. Zebrafish model derived from tumor cells of MM patients can be used as a tool for drug screening of MM.
