Effect of Long Non-Coding RNA LINC01268 on the Malignant Biological Behaviors of Acute Myeloid Leukemia Cells.
10.19746/j.cnki.issn.1009-2137.2023.06.002
- Author:
Wan CHEN
1
;
Ding-Yun GAN
2
Author Information
1. Department of Hematology & Endocrinology, Wuhan Third Hospital, Wuhan 430060, Hubei Province, China.
2. Department of Hematology & Endocrinology, Wuhan Third Hospital, Wuhan 430060, Hubei Province, China. E-mail: gandingyun@163.com.
- Publication Type:Journal Article
- Keywords:
LINC01268;
PI3K/AKT pathway;
acute myeloid leukemia;
biological behavior;
miR-217
- MeSH:
Humans;
Apoptosis;
bcl-2-Associated X Protein/metabolism*;
Caspase 3;
Cell Line, Tumor;
Cell Proliferation;
Leukemia, Myeloid, Acute/metabolism*;
MicroRNAs/metabolism*;
Phosphatidylinositol 3-Kinases/metabolism*;
Proto-Oncogene Proteins c-akt/metabolism*;
RNA, Long Noncoding/genetics*
- From:
Journal of Experimental Hematology
2023;31(6):1608-1616
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effect of long non-coding RNA LINC01268 on apoptosis of acute myeloid leukemia (AML) cells and related mechanisms.
METHODS:The expression levels of LINC01268 and miR-217 in peripheral blood samples from AML patients and AML cell lines HL-60 and KG-1 were detected by qRT-PCR. HL-60 cells were divided into pcDNA3.1-NC, pcDNA3.1-LINC01268, si-NC, si-LINC01268, miR-NC, miR-217 mimics, si-LINC01268 + inhibitor-NC and si-LINC01268+ miR-217 inhibitor groups. The mRNA expressions of LINC01268 and miR-217 were detected by qRT-PCR. The targeting relationship between LINC01268 and miR-217 was detected by dual-luciferase reporter assay. Cell viability was detected by CCK-8 assay. Cell cycle distribution and apoptosis were detected by flow cytometry. The expression of cell cycle and apoptosis-related proteins p21, Bcl-2, Bax, caspase-3 and PI3K/AKT signaling pathway-related proteins were detected by Western blot.
RESULTS:The expression of LINC01268 in peripheral blood samples of AML patients and AML cell lines HL-60 and KG-1 was increased (P < 0.05), and the expression of miR-217 was decreased (P < 0.05). Compared with si-NC group and miR-NC group, the viability of HL-60 cells was decreased in si-LINC01268 group and miR-217 mimics group (P < 0.05), the proportion of cells in G1 phase and apoptosis rate were increased (P < 0.05), the protein expression levels of p21, Bax and caspase-3 were increased (P < 0.05), while the protein expression level of Bcl-2 was decreased (P < 0.05). LINC01268 targeted and negatively regulated the expression of miR-217, and inhibiting the expression of miR-217 partially reversed the effects of LINC01268 interference on the viability, cell cycle and apoptosis of HL-60 cells. Interference with LINC01268 could inhibit the activity of PI3K/AKT signaling pathway. Inhibiting the expression of miR-217 could partially reverse the inhibition of LINC01268 interference on PI3K/AKT signaling pathway.
CONCLUSION:LINC01268 is highly expressed and miR-217 is lowly expressed in AML cells. LINC01268 can promote the activity of PI3K/AKT signaling pathway, increase the survival rate and inhibit the apoptosis of AML cells by targeting miR-217 expression.
